Microbiology: A Systems Approach
Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
bartleby

Concept explainers

Question
Book Icon
Chapter 21.4, Problem 9AYP
Summary Introduction

To discuss:

Reasons for the increase of pertussis cases over the past three decades.

Concept introduction:

Pertussis disease is caused by microorganism Bordetella pertussis which is a gram-negative coccobacillus bacterium. It has filamentous hemagglutinin on its surface which helps in adhering it to the surface of epithelial cells. It secretes pertussis cytotoxic and a tracheal cytotoxin. The layer of ciliated epithelial cells lining the respiratory tract is damaged.

Blurred answer
Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1)What is the selectable marker in this experiment? How…
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:9781305143531
Author:CURREN
Publisher:Cengage
Text book image
Body Structures & Functions Updated
Biology
ISBN:9780357191606
Author:Scott
Publisher:Cengage
Text book image
Body Structures & Functions
Biology
ISBN:9781285695495
Author:Scott
Publisher:Cengage
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Text book image
An Illustrated Guide To Vet Med Term
Biology
ISBN:9781305465763
Author:ROMICH
Publisher:Cengage
Text book image
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage