The identification of strain of resistant or non-resistant M. tuberculosis. Introduction: The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

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Answer 1

Question

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

Question

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

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Answer 3

Question

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

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Answer 4

Question

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

Expert Solution & Answer

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Answer 5

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

Answer 6

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Answer 7

Chapter 21.5, Problem 2CF

Summary Introduction

To determine:

The identification of strain of resistant or non-resistant M. tuberculosis.

Introduction:

The polymerase chain reaction is technique to amplify a piece of DNA very rapidly outside a living cell. It uses the same mechanism for multiplication of DNA as the cells use for DNA replication by using enzyme Taq polymerase (type of DNA polymerase).

Answer 8

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

Answer 9

Summary Introduction

To determine:

The precaution should be taken in homeless shelters, mental health facilities, and jails to prevent transmission of TB.

Introduction:

Tuberculosis is a potential fatal caused by Mycobacterium tuberculosis which can infect almost all part of the body, but it mainly infect the upper respiratory tract in humans. The disease can be treated with antibiotic and certain drugs.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 21.1 - Prob. 1AYP Ch. 21.1 - Prob. 2AYP Ch. 21.2 - Prob. 3AYP Ch. 21.3 - Prob. 4AYP Ch. 21.3 - Prob. 5AYP Ch. 21.3 - Prob. 6AYP Ch. 21.4 - Prob. 7AYP Ch. 21.4 - Prob. 8AYP Ch. 21.4 - Prob. 9AYP Ch. 21.4 - Prob. 10AYP

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