Chapter 21, Problem 21.1P

Interpretation Introduction

Interpretation:

Difference between eluent and eluate has to be given.

Concept Introduction:

Principle of chromatography is the mobility of analyte across two phase namely mobile and stationary phase. For effective separation of analyte, the sample may be required to dissolve in some gas or liquid /supercritical fluids referred to as mobile phases. Another phase used in the chromatographic analysis is the stationary phase often fixed as inert silica gel or as liquid silicon grease adhered to an inert solid support. Immiscible phase has got adsorption sites to retain analyte as it runs along with the mobile phase.

Explanation of Solution

Phenomenon that allows analyte injected within a chromatographic column to get separated when the mobile phase runs over a stationary solid phase is known as elution.

Two fluids, eluent, and eluate can be distinguished in the illustration as follows:

Hence, eluent may be defined as a fluid that enters from the top of the chromatographic column while fluid that is released from the other end is termed as an eluate.