Chapter 21, Problem 21.2P

Interpretation Introduction

Interpretation:

Of two columns with plate heights $\text{0}\text{.1 mm}$ and $\text{1 mm}$ one that can provide narrower peak separation has to be identified.

Concept Introduction:

Expression to compute number of theoretical plate is as follows:

  $N=\frac{5.55t_{\text{r}}{}^2}{w_{1/2}^2}$

Here,

$t_{\text{r}}$ denotes analyte’s  retention time.

$w_{1/2}$ denotes peak width at half-height.

$N$ denotes number of theoretical plate.

It has been found that greater the equilibration steps narrower will be band so this suggests $N$ should be high for more resolution or narrower separation.

Expression to compute plate height is as follows:

  $H=\frac{L}{N}$

Here,

$N$ denotes number of theoretical plate.

$L$ denotes length of chromatographic column.

$H$ denotes plate height.