Exploring Chemical Analysis
Exploring Chemical Analysis
5th Edition
ISBN: 9781429275033
Author: Daniel C. Harris
Publisher: Macmillan Higher Education
Question
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Chapter 21, Problem 21.3P

(a)

Interpretation Introduction

Interpretation:

Reason behind the broader band after 26 min has to be given.

Concept Introduction:

Principle of chromatography is the mobility of analyte across two phases namely mobile and stationary phase. For effective separation of the analyte, the sample may be required to dissolve in some gas or liquid /supercritical fluids referred to as mobile phases. Another phase used in the chromatographic analysis is the stationary phase often fixed as inert silica gel or as liquid silicon grease adhered to an inert solid support. Immiscible phase has got adsorption sites to retain analyte as it runs along with the mobile phase.

Solute molecules within analyte diffuse away from the center bilaterally. This is essentially a slow-paced equilibration of solute across the mobile and stationary phases.

(a)

Interpretation Introduction

Interpretation:

Reason behind the best separation in chromatographic separation achieved when an optimum flow rate occurs has to be given.

Concept Introduction:

Refer to part (a).

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