Concept explainers
(a) Interpretation:
Identify the parts of the C0t curve that correcspond to anealing of (1) unique sequences, (2) modereately repetetive sequence, and (3) highly repetetive sequences.
Introduction:
The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics.
Pictorial representation:
The given below is the C0t analysis, in which the types of DNA sequences are labeled:
(b) Interpretation:
Identify the parts of the C0t curve that correcspond to anealing single copy gene.
Introduction:
The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics. The single copy genes are the unique sequences.
Pictorial representation:
The given below is the C0t analysis, in which the types of DNA renaturation obtained is labeled:
(c) Interpretation:
The difference in the annealing activity of the cDNA and normal ovalbumin.
Introduction:
The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics.
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Biochemistry: Concepts and Connections (2nd Edition)
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