Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 21, Problem 17P
Interpretation Introduction

(a) Interpretation:

Identify the parts of the C0t curve that correcspond to anealing of (1) unique sequences, (2) modereately repetetive sequence, and (3) highly repetetive sequences.

Introduction:

The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics.

Pictorial representation:

The given below is the C0t analysis, in which the types of DNA sequences are labeled:

Biochemistry: Concepts and Connections (2nd Edition), Chapter 21, Problem 17P , additional homework tip  1

Interpretation Introduction

(b) Interpretation:

Identify the parts of the C0t curve that correcspond to anealing single copy gene.

Introduction:

The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics. The single copy genes are the unique sequences.

Pictorial representation:

The given below is the C0t analysis, in which the types of DNA renaturation obtained is labeled:

Biochemistry: Concepts and Connections (2nd Edition), Chapter 21, Problem 17P , additional homework tip  2

Interpretation Introduction

(c) Interpretation:

The difference in the annealing activity of the cDNA and normal ovalbumin.

Introduction:

The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics.

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