Concept explainers
Interpretation: The exponential nature of PCR allows spectacular increases in the abundance of a DNA sequence being amplified. Consider a
The fraction of the genome represented by this sequence should be determined. The fractional abundance of this sequence in this genome should be identified.
The fractional abundance of this target sequence after
Concept introduction:
The method of making of copies of specific region of DNA uses polymerase chain reaction or PCR. The amount of target sequence in the reaction is doubled with each PCR cycle. Example: ten cycles will theoretically multiply the DNA segment by a factor of about one thousand and so on.
Explanation of Solution
A
The sequence is
The PCR amplification doubles the number of copies each time. Amplification is
So, after
Thus, after
After
Thus, after
After
Thus, after
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Chapter 21 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
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