(a) Interpretation: Explain why the recombinant plasmid is treated with EcoRI to determine its size. Concept introduction: Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

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Answer 1

Question

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

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Answer 2

Question

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

Expert Solution & Answer

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See solution

Answer 3

Question

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

Expert Solution & Answer

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See solution

Answer 4

Question

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

Expert Solution & Answer

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Answer 5

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

Answer 6

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Answer 7

Chapter 21, Problem 4P

Interpretation Introduction

(a) Interpretation:

Explain why the recombinant plasmid is treated with EcoRI to determine its size.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Answer 8

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Answer 9

Interpretation Introduction

(b) Interpretation:

The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Answer 10

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

Answer 11

Interpretation Introduction

(c) Interpretation:

The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.

Concept introduction:

Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.

Pictorial representation:

Answer 12

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Answer 13

Expert Solution & Answer

Answer 14

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Answer 15

Ch. 21 - Prob. 1P Ch. 21 - Prob. 2P Ch. 21 - Prob. 3P Ch. 21 - Prob. 4P Ch. 21 - Prob. 5P Ch. 21 - Prob. 6P Ch. 21 - Prob. 7P Ch. 21 - Prob. 8P Ch. 21 - Prob. 9P Ch. 21 - Prob. 10P

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