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Concept explainers
(a) Interpretation:
Explain why the recombinant plasmid is treated with EcoRI to determine its size.
Concept introduction:
Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.
(b) Interpretation:
The discrepancy between the size of the recombinant molecule and the sum of the size of the HindIII cleavage fragments should be explained.
Concept introduction:
Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.
(c) Interpretation:
The diagram of the recombinant showing the locations of the HindIII cleavage sites should be drawn.
Concept introduction:
Plasmids are circular and small double stranded DNA and is different from the DNA of the cell's chromosome. Plasmids are naturally found in bacterial calls and some Eukaryotes. They act as a tool for the purpose of cloning, transfer and gene manipulation. Plasmids used for experimental purposes are termed as vectors. The genes or DNA fragments can be inserted into the plasmid vector which thus forms recombinant plasmid.
Pictorial representation:
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Chapter 21 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
- 14. What is the IUPAC name of this compound? A) 6-hydroxy-4-oxohexanenitrile B) 5-cyano-3-oxo-1-pentanol C) 5-cyano-1-hydroxy-3-pentanone D) 1-cyano-5-hydroxy-3-pentanone E) 5-hydroxy-3-oxopentanenitrile HO. CNarrow_forward13. What is the IUPAC name of this compound? A) 5-hydroxy-3,3-dimethylpentanoic acid B) 3,3-dimethylpentanoic acid C) 3,3-dimethyl-1-oxo-1,5-pentanediol D) 1,5-dihydroxy-3,3-dimethylpentanal E) 4-hydroxy-2,2-dimethylbutanoic acid HO OHarrow_forwardHelp me understand how carbon disulfide leads to toxicity in the brain, using terms like distal axonopathy, neurofilaments, covalent cross-linking, adducts, etc.,...please intuitively explain what is happening and where and the effects of it. For example, I know that CS2 reacts with amide and sulfhydryl groups on proteins, but what proteins exactly and where are they located?arrow_forward
- What is the standard free energy change (in kJ/mole) of the spontaneous reaction between Oxygen and NADH to form H2O2 and NAD+?arrow_forwardRedox Chemistry: Give standard free energy changes expected for the following reactions:-Succinate -> fumarate (using FAD/FADH2)-Oxaloacetate -> Malate (using NAD/NADH)-NADH --> NAD+ (using FMN/FMNH2)-CoQ --> CoQH2 (using Cytochrome C)arrow_forwardGive examples of balanced redox reactions that match the following:-Catabolic-Anabolic-Oxidative-Reductivearrow_forward
- If there are 20uM of a GLUT2 transporter on the surface of a cell, each able to move 8 per second, and 50mM glucose outside of the cell, what is the flux into the cell in mM/sec?arrow_forwardA transporter is responsible for antiporting calcium and glucose. The transporter brings glucose into the cell and sends calcium out of the cell. If blood [calcium] = 2.55mM and intracellular [calcium] = 7uM, blood [glucose] = 5.2mM, and intracellular [glucose] = 40uM, what is the free energy of transport? Assume a membrane potential of 62mV (negative inside).arrow_forwardAn ATP-coupled transporter is used to import 1 phosphate from the extracellular environment. Intracellular phosphate exists at 65mM, while it is 2mM outside.Assume a free energy change of ATP hydrolysis of -42.7 kJ/mol. What is the net free energy change of the coupled reaction? Assume a membrane potential of 70mV.arrow_forward
- Another transporter brings 3 chloride ions into the cell. Outside, chloride has a concentration of 107mM, and 4mM inside the cell. Assuming a membrane potential of 62mV (negative inside), what is the free energy of transport of these ions?arrow_forwardFor the Oxaloacetate -> Malate reaction, assume the normal ratio of NAD/NADH, what is the maximum ratio of Malate/Oxaloacetate that will allow reaction progress?arrow_forwardA particular particle is trying to cross a membrane by simple diffusion from a high concentration of 20mM to a low concentration of 20uM. If a membrane is 15uM in width, and the diffusion coefficient of the particle is 5 uM/sec, what is the influx in uM/sec?arrow_forward
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