To draw: The complementary strand of the given sequence. Concept introduction: DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA .

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Answer 1

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Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

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19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

Expert Solution & Answer

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Answer 3

Question

Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

Expert Solution & Answer

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Answer 4

Question

Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

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Answer 5

Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

Answer 6

Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Answer 7

Chapter 20.1, Problem 2CC

Summary Introduction

To draw: The complementary strand of the given sequence.

Concept introduction:

DNA is a double stranded molecule consisting of two strands of nucleotides. The bases in one strand are complementary to the bases on the other strand. If the sequence of one DNA strand is known, the sequence of its complementary strand can be predicted. This is known as complementarity of DNA.

Answer 8

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

Answer 9

Summary Introduction

To determine: If the above given DNA molecule can be cut by Pvu I restriction enzyme, and if yes, draw the digested products.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. The restriction enzyme Pvu I recognizes a specific sequence. These make staggered cuts at or adjacent to their specific recognition site and produce sticky ends.

Answer 10

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Answer 11

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Answer 12

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Answer 13

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