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To explain: The role of complementary base pairing during RT-PCR.
Concept introduction: The gene functions can be studied through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to the gene of interest are to be identified. The methods used for these include RT-PCR, DNA microarray analysis, and RNA sequencing. These are based on the base-pairing between the nucleotides.
To explain: The role of complementary base pairing during microarray analysis.
Concept introduction: DNA microarray analysis is a technique used in molecular biology for identifying the expression levels of genes where collection of microscopic DNA spots is attached to a solid surface known as DNA chip.
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Chapter 20 Solutions
Campbell Biology & Alkaline Vents Supplemental Materials for Campbell Biology & Mastering Biology with Pearson eText -- ValuePack Access Card -- for Campbell Biology Package
- 4.arrow_forward2arrow_forward1. 2. 3. Marine fish cells are hypotonic compared to their seawater environment; their cells lose water by osmosis and gain solutes. If you add heterotrophic respiration and autotrophic respiration together and then subtract that value from gross primary productivity, then you have a more refined estimate of ecosystem carbon storage than NEE. Differential heating due to the earth's tilt generates the global wind AND oceanic circulation patternsarrow_forward
- KD 200- 116- 66- Vec ATF6 (670) ATF6 (402) ATF6 (373) ATF6 (366) I I 45- 1 2 3 4 5 ATFG (360) (e/c) 9V ATFG (402) g ant- ATF anti-KDEL DAPI barrow_forwardWestern blot results: what information can you get? Presence of proteins of your interest Levels of protein expression Levels of protein activation (must use activation state-specific antibody) Decreased function of the ATM kinase in aging mice. A C57BL/6 female 6 month Con IR 20 month C57BL/6 male 6 month 28 month Con IR Con IR Con IR p-ATM (S1981) ATM P-p53 (ser18) Actinarrow_forwardDoes it show the level of proteins? What about the amount? Levels of protein activation? How can you tell? Does the thickness tell you anything? What about the number of the lines?arrow_forward
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