FUNDAMENTALS OF BIOCHEMISTRY - LL FD
5th Edition
ISBN: 9781119598022
Author: Voet
Publisher: WILEY
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 20, Problem 7E
Summary Introduction
To explain: The reason why individuals with a hereditary deficiency of carnitine palmitoyl transferase II have muscle weakness.
Concept introduction: Carnitine palmitoyl transferase II is a mitochondrial enzyme that is encoded by CPT2 gene in the humans. CPT2 precursor is a mitochondrial membrane protein. It is transported to the mitochondrial membrane protein. The defect that occurs due to this CPT2 gene is called as carnitine palmitoyl transferase II deficiency.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 20 Solutions
FUNDAMENTALS OF BIOCHEMISTRY - LL FD
Ch. 20 - Prob. 1ECh. 20 - 2. Identify die products generated by the action...Ch. 20 - 3. (a) What is. the charge of bile acids in the...Ch. 20 - 4. Bacteria that live in the large intestine...Ch. 20 - Prob. 5ECh. 20 - 6. Adipocytes store fat in phospholipid-coaled...Ch. 20 - Prob. 7ECh. 20 - Prob. 8ECh. 20 - 9. The first three steps of ? oxidation (Fig....Ch. 20 - Prob. 10E
Ch. 20 - Prob. 11ECh. 20 - Prob. 12ECh. 20 - Prob. 13ECh. 20 - 14. Short chain fatty acids such as butyrate are...Ch. 20 - 15. Why are unsaturated fats preferable to...Ch. 20 - 16. Calculate the ATP yield for the complete...Ch. 20 - 17. Explain why the degradation of odd-chain fatty...Ch. 20 - Prob. 18ECh. 20 - 19. Why is it important that liver cells lack...Ch. 20 - 20. An animal is fed palmitate with a 14C-labeled...Ch. 20 - 21. On what cartoon atoms docs the 14CO2 used to...Ch. 20 - Prob. 22ECh. 20 - 23. Explain why adipocytes need glucose as well as...Ch. 20 - Prob. 24ECh. 20 - 25. Pharmacological inhibition of acetyl-CoA...Ch. 20 - 26. Explain why inhibiting the activity of...Ch. 20 - Prob. 27ECh. 20 - 28. An animal is fed palmitate with a 14C-labeled...Ch. 20 - Prob. 29ECh. 20 - Prob. 30ECh. 20 - Prob. 31CQCh. 20 - Prob. 32CQCh. 20 - 33. Digestion of plant materials generates...Ch. 20 - Prob. 34CQCh. 20 - 35. The tricarboxylate transport system supplies...Ch. 20 - Prob. 36CQCh. 20 - 37. Compare the energy cost, in ATP equivalents,...Ch. 20 - Prob. 38CQCh. 20 - Prob. 39CQCh. 20 - Prob. 40CQCh. 20 - Prob. 41CQCh. 20 - Prob. 42CQCh. 20 - Prob. 43CQCh. 20 - Prob. 1MTE
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Which type of enzyme catalyses the following reaction? oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase.arrow_forward+NH+ CO₂ +P H₂N + ATP H₂N NH₂ +ADParrow_forwardWhich type of enzyme catalyses the following reaction? oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase.arrow_forward
- Which features of the curves in Figure 30-2 indicates that the enzyme is not consumed in the overall reaction? ES is lower in energy that E + S and EP is lower in energy than E + P. What does this tell you about the stability of ES versus E + S and EP versus E + P.arrow_forwardLooking at the figure 30-5 what intermolecular forces are present between the substrate and the enzyme and the substrate and cofactors.arrow_forwardprovide short answers to the followings Urgent!arrow_forward
- Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvatedehydrogenase complex, resulting in acetyl-CoA and CO2. Provide a full mechanismfor this reaction utilizing the TPP cofactor. Include the roles of all cofactors.arrow_forwardB- Vitamins are converted readily into important metabolic cofactors. Deficiency inany one of them has serious side effects. a. The disease beriberi results from a vitamin B 1 (Thiamine) deficiency and ischaracterized by cardiac and neurological symptoms. One key diagnostic forthis disease is an increased level of pyruvate and α-ketoglutarate in thebloodstream. How does this vitamin deficiency lead to increased serumlevels of these factors? b. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 5 deficiency? c. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 2 /B 3 deficiency?arrow_forwardDraw the Krebs Cycle and show the entry points for the amino acids Alanine,Glutamic Acid, Asparagine, and Valine into the Krebs Cycle - (Draw the Mechanism). How many rounds of Krebs will be required to waste all Carbons of Glutamic Acidas CO2?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. Freeman
Lehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON