FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
5th Edition
ISBN: 9781119498742
Author: Voet
Publisher: WILEY
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Chapter 2, Problem 27CQ
Summary Introduction
To explain: What happens when tomato juice spills on a marble slab in chemical terms.
Concept introduction: Tomato is a sour fruit with full of nutrients. It contains many organic acids, which are the cause of the sour taste. In tomato, the organic acids are the citric acid, malic acid, and other dicarboxylic amino acids. Marble slab is a sedimentary rock contains carbonate and lime stone.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 2 Solutions
FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
Ch. 2 - Prob. 1ECh. 2 - 2. Rank the water solubility of the following...Ch. 2 - 3. Approximately how many molecules of H2O are in...Ch. 2 - 4. An E. coli cell contains about 2.6 × 108 ions,...Ch. 2 - Prob. 5ECh. 2 - Prob. 6ECh. 2 - 7. Describe what happens when a dialysis ban...Ch. 2 - 8. A red blood cell his an internal salt...Ch. 2 - 9. Draw the structures of the conjugate bases of...Ch. 2 - Prob. 10E
Ch. 2 - 11. Indicate the ionic species of ammonia that...Ch. 2 - 12. Indicate the ionic species of phosphoric acid...Ch. 2 - 13. Calculate the pH of a 200 mL solution of pure...Ch. 2 - 14. Calculate the pH of a 1 L solution containing...Ch. 2 - 15. A solution is nude by mixing 50 mL of 2.0 M...Ch. 2 - 16. What is the pK of the weak acid HA if a...Ch. 2 - 17. (a) At any instant, how many water molecules...Ch. 2 - 18.
Would phosphoric acid or succinic acid be a...Ch. 2 - 19. Use Coulomb’s law (Equation 2-1) to explain...Ch. 2 - Prob. 20CQCh. 2 - 21. Certain C—H groups can form weak hydrogen...Ch. 2 - 22. Explain why water forms nearly spherical...Ch. 2 - Prob. 23CQCh. 2 - 24. You have a 5 ml. sample of a protein in 0.5 M...Ch. 2 - 25. Patients with kidney failure frequently...Ch. 2 - 26. The kidneys function to eliminate ammonia from...Ch. 2 - Prob. 27CQCh. 2 - Prob. 28CQCh. 2 - 29. How many grams of sodium succinate (formula...Ch. 2 - 30. Estimate the volume of a solution of 5 M NaOH...Ch. 2 - 31. You need a buffer at pH 7.5 for use in...Ch. 2 - Prob. 32CQCh. 2 - MORE TO EXPLORE Compare and contrast the...
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