Fundamentals of Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134396026
Author: Frederic H. Martini, Judi L. Nath, Edwin F. Bartholomew
Publisher: PEARSON
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Chapter 2, Problem 25RQ
What is a salt? How does a salt differ from an acid or a base?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
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oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 2 Solutions
Fundamentals of Anatomy & Physiology (11th Edition)
Ch. 2 - Define atom.Ch. 2 - Atoms of the same element that have different...Ch. 2 - How is it possible for two samples of hydrogen to...Ch. 2 - Prob. 4CPCh. 2 - Prob. 5CPCh. 2 - Both oxygen and neon are gases at room temperature...Ch. 2 - Prob. 7CPCh. 2 - Prob. 8CPCh. 2 - Prob. 9CPCh. 2 - Prob. 10CP
Ch. 2 - What is an enzyme?Ch. 2 - Prob. 12CPCh. 2 - Prob. 13CPCh. 2 - Explain how the chemical properties of water make...Ch. 2 - Define pH, and explain how the pH scale relates to...Ch. 2 - What is the significance of pH in physiological...Ch. 2 - Define the following terms: acid, base, and salt.Ch. 2 - Prob. 18CPCh. 2 - Prob. 19CPCh. 2 - Which functional group acts as an acid?Ch. 2 - Prob. 21CPCh. 2 - Describe lipids.Ch. 2 - Prob. 23CPCh. 2 - Prob. 24CPCh. 2 - Prob. 25CPCh. 2 - Describe a nucleic acid.Ch. 2 - Prob. 27CPCh. 2 - Describe ATP.Ch. 2 - What molecule is produced by the phosphorylation...Ch. 2 - An oxygen atom has eight protons (a) Sketch in the...Ch. 2 - What is the following type of decomposition...Ch. 2 - The subatomic particle with the least mass (a)...Ch. 2 - Isotopes of an element differ from each other in...Ch. 2 - The number and arrangement of electrons in an...Ch. 2 - All organic compounds in the human body contain...Ch. 2 - A substance containing atoms of different elements...Ch. 2 - All the chemical reactions that occur in the human...Ch. 2 - Which of the following chemical equations...Ch. 2 - Prob. 10RQCh. 2 - A pH of 7.8 in the human body typifies a condition...Ch. 2 - A(n) _____ is a solute that dissociates to release...Ch. 2 - Special catalytic molecules called _____ speed up...Ch. 2 - Which of the following is not a function of a...Ch. 2 - Complementary base pairing in DNA includes the...Ch. 2 - What are the three subatomic panicles in atoms?Ch. 2 - Prob. 17RQCh. 2 - Prob. 18RQCh. 2 - List seven major functions performed by proteins.Ch. 2 - (a) What three basic components make up a...Ch. 2 - What three components are required to create the...Ch. 2 - If a polypeptide contains 10 peptide bonds, how...Ch. 2 - Prob. 23RQCh. 2 - Prob. 24RQCh. 2 - What is a salt? How does a salt differ from an...Ch. 2 - Prob. 26RQCh. 2 - In an exergonic reaction, (a) large molecules are...Ch. 2 - Prob. 28RQCh. 2 - Prob. 29RQCh. 2 - An atom of the element calcium has 20 protons and...Ch. 2 - A certain reaction pathway consists of four steps....Ch. 2 - Prob. 32RQCh. 2 - Prob. 1CCCh. 2 - Prob. 2CC
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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