The tracking of intracellular molecules by super-resolution imaging. Introduction: Super-resolution imaging is the process by which intracellular molecules can be tracked live inside a living system especially a bacterial cell. The process of transport of macromolecules during intracellular events can be easily tracked with the help of specific dyes that can bind to target molecules. The properties of these dyes to absorb and emit light at different wavelengths in visible light can help track many intracellular events. Super-resolution imaging combines the properties of fluorescent color absorbing dyes and emitting dyes to track live intracellular events like cell division, deoxyribose nucleic acid ( DNA) replication. The dye binds to the target molecule and its movement is tracked by the fluorophore emission spectrum. This is based on peak position movements as light from each point appears as a narrow peak. There is an uncertainty of the exact peak position of ten percent yet the tracking of a single protein bound to a fluorophore could be obtained. Another application of this method is the use of green and yellow fluorescent dye to track two key proteins par A and par C involved in bacterial transition between stalked and flagella cells. The movement of the fluorescence across the cell can be tracked because of the different colored dyes confirming the role of the two proteins in the process by live tracking. The emission of light is also not uniform in case fluorophore emits a light.

Question

Want to see the full answer?

Answer 1

Question

Chapter 2, Problem 14RQ

Summary Introduction

To review:

The tracking of intracellular molecules by super-resolution imaging.

Introduction:

Super-resolution imaging is the process by which intracellular molecules can be tracked live inside a living system especially a bacterial cell. The process of transport of macromolecules during intracellular events can be easily tracked with the help of specific dyes that can bind to target molecules. The properties of these dyes to absorb and emit light at different wavelengths in visible light can help track many intracellular events.

Super-resolution imaging combines the properties of fluorescent color absorbing dyes and emitting dyes to track live intracellular events like cell division, deoxyribose nucleic acid (DNA) replication. The dye binds to the target molecule and its movement is tracked by the fluorophore emission spectrum. This is based on peak position movements as light from each point appears as a narrow peak. There is an uncertainty of the exact peak position of ten percent yet the tracking of a single protein bound to a fluorophore could be obtained.

Another application of this method is the use of green and yellow fluorescent dye to track two key proteins par A and par C involved in bacterial transition between stalked and flagella cells. The movement of the fluorescence across the cell can be tracked because of the different colored dyes confirming the role of the two proteins in the process by live tracking. The emission of light is also not uniform in case fluorophore emits a light.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

None

Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here: