Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Textbook Question
Chapter 2, Problem 13TYU
FOCUS ON ORGANIZATION
Several emergent properties of water contribute to the suitability of the environment for life. In a short essay (100-150 words), describe how the ability of water to function as a versatile solvent arises from the structure of water molecules.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 2 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 2.1 - Is a trace element an essenteal element? Explain.Ch. 2.1 - Prob. 2CCCh. 2.2 - A nitrogen atom has 7 protons and the most common...Ch. 2.2 - Prob. 2CCCh. 2.2 - Prob. 3CCCh. 2.3 - Why does the structure HCCH fail to make sense...Ch. 2.3 - What holds the atoms together in a crystal of...Ch. 2.3 - If you were a phamaceutical researcher, why would...Ch. 2.4 - Which type of chemical reaction occurs faster at...Ch. 2.4 - Write an equation that uses the products of...
Ch. 2.5 - Prob. 1CCCh. 2.5 - How can the freezing of water crack boulders?Ch. 2.5 - Compared with a basic solution at pH 9, the same...Ch. 2.5 - What would be the effect on the properties of the...Ch. 2.5 - Prob. 5CCCh. 2 - The reactivity of an atom arises from A. the...Ch. 2 - Which of the following statements correctly...Ch. 2 - Many mammals control their body temperature by...Ch. 2 - We can be sure that a mole of table sugar and a...Ch. 2 - Measurements show that the pH of a particular lake...Ch. 2 - Prob. 6TYUCh. 2 - What coefficients must be placed in the following...Ch. 2 - A Slice of pizza has 500 kcal. If we could burn...Ch. 2 - Draw the hydration shells that from around a...Ch. 2 - What do climate change (see Concept 1.1) and ocean...Ch. 2 - SCIENTIFIC INQUIRY Female luna moths (Actias luna)...Ch. 2 - FOCUS ON EVOLUTION The percentage of naturally...Ch. 2 - FOCUS ON ORGANIZATION Several emergent properties...Ch. 2 - SYNTHESIZE YOUR KNOWLEDGE How do cats drink?...
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