Genetics
Genetics
5th Edition
ISBN: 9781464109461
Author: Benjamin A. Pierce
Publisher: MAC HIGHER
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Chapter 19.4, Problem 8CC
Summary Introduction

Introduction:

Sanger’s method or dideoxy sequencing is a method of gene sequencing. It is also known by the name of chain termination method. This method required a single stranded DNA template, DNA primer, DNA polymerase, dNTPs (Deoxynucleoside Triphosphates) and ddNTPs (Di-Deoxynucleotide Triphosphates).

Single stranded DNA template is used to synthesize complimentary copy of DNA with the help of DNA polymerase in the presence of nucleotides. DNA polymerase utilizes ddNTPs as nucleotide, and it gets included into the growing chain, but chain elongation stops at ddNTPs due to absence of 3'-OH group. In this, 4 different ddNTPs (A, T, G, and C) are used and they take 4 separate reactions.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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