The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel. Introduction: EcoRI is type II restriction enzyme. 5 ' − GAATTC − 3 ' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide .

Question

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Answer 1

Question

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.

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Answer 2

Question

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.

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Answer 3

Question

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.

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Answer 4

Question

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.

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Answer 5

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.

Answer 6

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

Answer 7

Chapter 19.2, Problem 30AQP

a.

Summary Introduction

To determine:

The piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

Answer 8

a.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel shows that DNA fragments are present after the EcoRI has cleaved at 2 kb, 4 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 1

Fig. 1: Gel electrophoresis DNA fragmentation.

After the action of enzyme EcoRI on the DNA, it will produce three fragments of length 2 kb, 4 kb and 5 kb. These bands will appear on the gel as shown in the Fig.1.

Answer 9

a.

Expert Solution

Answer 10

a.

Expert Solution

Answer 11

Expert Solution

Answer 12

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

Answer 13

b.

Summary Introduction

To determine:

If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that drew in part a?

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

Answer 14

b.

Expert Solution

Explanation of Solution

Pictorial representation: The following gel on that DNA fragments only two fragments of length is obtained 6 kb and 8 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 2

Fig 2 Gel fragmentation

Only two fragments will obtained if the EcoRI site 1 is mutated, two fragments are 6 and 5 kb.

Answer 15

b.

Expert Solution

Answer 16

b.

Expert Solution

Answer 17

Expert Solution

Answer 18

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

Answer 19

c.

Summary Introduction

To determine:

The difference of banding pattern on the gel that drew in part a if mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

Answer 20

c.

Expert Solution

Explanation of Solution

Pictorial representation: The gel electrophoresis after the mutation occurs at both sites.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 3

Fig. 3: Gel electrophoresis result

If mutation occur on the both sites, then there will be no cutting and if there will be no cutting then fragment will remain same.

Answer 21

c.

Expert Solution

Answer 22

c.

Expert Solution

Answer 23

Expert Solution

Answer 24

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

Answer 25

d.

Summary Introduction

To determine:

The difference of banding pattern on the gel differ from the one that drew in part a if the 1000 bp of DNA were inserted between the two restriction sites.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

Answer 26

d.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only two fragments of length is obtained 2 kb and 5 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 4

Fig 4: Gel fragmentation

Inserting 1000 kb of DNA between the restriction sites will increase the 4 kb fragment to 5 kb fragment, then two fragment 5 and 2 kb length will come, as shown in the fig 4.

Answer 27

d.

Expert Solution

Answer 28

d.

Expert Solution

Answer 29

Expert Solution

Answer 30

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.

Answer 31

e.

Summary Introduction

To determine:

The difference in the banding pattern on the gel differ from the one that drew in part a if 500 bp of DNA between the two restriction sites were deleted.

Introduction:

EcoRI is type II restriction enzyme. 5'−GAATTC−3' is the recognition site of EcoRI and it is obtained from the bacterium Escherichia coli. It is type II enzyme that cut at specific site. Gel electrophoresis is the technique used for the separation of molecule in the gel under the influence of current. Smaller the molecule, easily does it pass through the pores. Ethidium bromide is staining dye, which stacks in the bases of nucleotide.

Answer 32

e.

Expert Solution

Explanation of Solution

Pictorial representation: The gel on that DNA fragments only three fragments of length are obtained 5 kb, 3.5 kb and 2 kb.

Genetics, Chapter 19.2, Problem 30AQP , additional homework tip 5

Fig 5 Gel fragmentation

Deleting 500 kb of DNA between the two restriction sites will reduce the size of the fragment, results three fragment obtained 5 kb, 3.5 kb and 2 kb, and they are shown in the fig 5.