Chapter 19.3, Problem 14COQ

Summary Introduction

To explain:

In situ hybridization and application of this technique.

Introduction:

In situ hybridization (ISH) is a technique used to detect the location of specific DNA, RNA within the cells, chromosome, and give the information. It is type of hybridization that uses a labeled complementary probe to locate the location of DNA, RNA sequence in the cell or chromosome. There are two type of ISH: fluorescence in situ hybridization (FISH) or chromogenic in situ hybridization (CISH) application of ISH is: prenatal test during pregnancy to check the chromosomal abnormalities, karotyping and genetic analysis.