The way by which polymerase chain reaction is used to amplify specific DNA sequence. Introduction: PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2 + ions, and buffer.

Question

Want to see the full answer?

Answer 1

Question

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Hair follicle formation is thought to result from a reaction-diffusion mechanism with Wnt and its antagonist Dkk1. How is Dkk1 regulated by Wnt? Describe specific cis-regulatory elements and the net effect on Dkk1 expression.

Limetown S1E4 Transcript: E n 2025SP-BIO-111-PSNT1: Natu X Natural Selection in insects X + newconnect.mheducation.com/student/todo CA NATURAL SELECTION NATURAL SELECTION IN INSECTS (HARDY-WEINBERG LAW) INTRODUCTION LABORATORY SIMULATION A Lab Data Is this the correct allele frequency? Is this the correct genotype frequency? Is this the correct phenotype frequency? Total 1000 Phenotype Frequency Typica Carbonaria Allele Frequency 9 P 635 823 968 1118 1435 Color Initial Frequency Light 0.25 Dark 0.75 Frequency Gs 0.02 Allele Initial Allele Frequency Gs Allele Frequency d 0.50 0 D 0.50 0 Genotype Frequency Moths Genotype Color Moths Released Initial Frequency Frequency G5 Number of Moths Gs NC - X

Which of the following is not a sequence-specific DNA binding protein? 1. the catabolite-activated protein 2. the trp repressor protein 3. the flowering locus C protein 4. the flowering locus D protein 5. GAL4 6. all of the above are sequence-specific DNA binding proteins

Answer 2

Question

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Answer 6

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Answer 7

Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg 2+ ions, and buffer.

Answer 8

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Answer 9

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.

Answer 10

Expert Solution & Answer

Answer 11

Expert Solution & Answer

Answer 12

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 13

Ch. 19.1 - Prob. 1COQ Ch. 19.2 - Prob. 10COQ Ch. 19.2 - Prob. 11COQ Ch. 19.2 - Prob. 1CC Ch. 19.2 - Prob. 25AQP Ch. 19.2 - Prob. 26AQP Ch. 19.2 - Prob. 27AQP Ch. 19.2 - Prob. 28AQP Ch. 19.2 - Prob. 29AQP Ch. 19.2 - Prob. 2CC

Want to see the full answer?

Chapter 19 Solutions