Chapter 19.2, Problem 10COQ

Summary Introduction

To explain:

The way by which polymerase chain reaction is used to amplify specific DNA sequence.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is having three main steps that are denaturing, annealing, and extension. PCR reaction contains template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg ${}^{2+}$ ions, and buffer.

Summary Introduction

To determine:

The limitation of the PCR.

Introduction:

PCR is polymerase chain reaction, which make many copies of DNA from a single copy of DNA. It takes place in 3 steps: denaturation, annealing, and extension. PCR is fast, accurate and best method to amplify DNA. It has some limitation like, taq polymerase enzyme is unable to carry out the process of proof-reading, and it only makes copies up to 20,000 base pair.