Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 19, Problem 8TYK
Summary Introduction
Introduction: A virus is an infectious agent that cannot be seen through the naked eyes. Virology is the study of viruses and virologists are the biologists who study viruses. Viruses are obligate intracellular
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has been assembled by researchers and transplanted into a donor bacterial strain to study never
before seen gene functions.
Select one:
a Transgenic genome
b. Recombinant DNA sequence
C. Knockdown gene
d. Synthetic genome
e. Recombinant plasmid x
Proteins that bind to a specific DNA sequence and help control the recruitment of
RNA polymerase are called ...
1.) CRISPR/Cas9
2.) restriction enzymes
3.) transcription factors
4.) topoisomerases
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
Chapter 19 Solutions
Study Guide for Campbell Biology
Ch. 19 - In the following diagram of lytic and lysogenic...Ch. 19 - Summarize the flow of genetic information during...Ch. 19 - How does a virus spread throughout a plant? Are...Ch. 19 - Create a concept map that describes the lytic and...Ch. 19 - Prob. 2SYKCh. 19 - Prob. 1TYKCh. 19 - Prob. 2TYKCh. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - T2 and T4 are two types of bacteriophage that...
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- Plasmids are important in biotechnology because they are Group of answer choices a. a vehicle for the insertion of foreign genes into bacteria b. recognition sites on recombinant DNA strands c. surfaces for protein synthesis in eukaryotic recombinants d. viruses incorporated into the host DNAarrow_forwardWhich of the following is a primary participant in cloning an isolated gene? a. restriction endonuclease b. vector c. host organism d. all of thesearrow_forwardWhat carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forward
- The function of CRISPR gene cluster in Streptococcus pyogenes is to Select one or more: a. destroy phage DNA in the bacteria. O b. protect phage DNA in the bacteria. Oc. repair the damaged DNA in its genome. O d. delete the specific genes in its genome.arrow_forwardBacteria are frequently used in the generation of recombinant DNA molecules to: Group of answer choices A) Make copies of the vector + insert plasmid B) Ligate the insert into the vector C) Digest the vector for insert ligation D) Amplify the insert by PCRarrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forward
- Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forward
- a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?arrow_forwardWhich of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forwardWhat is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forward
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