Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Textbook Question
Chapter 19, Problem 12TYK
The CRISPR-Cas system allows a bacterium to
- a. degrade DNA from viruses that it has previously been exposed to.
- b. degrade DNA from viruses that it has never been exposed to.
- c. prevent phages from entering the cell.
- d. prevent prophages from exiting the bacterial chromosome.
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If the HIV did not have a reverse transcriptase enzyme, then which of the following would apply?
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a. destroy phage DNA in the bacteria.
O b. protect phage DNA in the bacteria.
Oc. repair the damaged DNA in its genome.
O d. delete the specific genes in its genome.
In Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make?
(photo linked)
A.
Protein does not enter the bacterium during transduction.
B.
DNA does not stay in the media after transduction.
C.
All of the phage DNA was radiolabeled.
D.
Bacteria can be transduced by phage.
Chapter 19 Solutions
Study Guide for Campbell Biology
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- Which of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardWhich nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.arrow_forwardWhich of the following would be the first step in biosynthesis of a virus with a - (minus) strand of RNA? A. Synthesis of double stranded RNA from a DNA template B. Transcription of mRNA from DNA C. Synthesis of DNA from an RNA template D. Synthesis of double stranded RNA from an RNA template E. Synthesis of DNA from a DNA templatearrow_forward
- Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionarrow_forwardProteins that bind to a specific DNA sequence and help control the recruitment of RNA polymerase are called ... 1.) CRISPR/Cas9 2.) restriction enzymes 3.) transcription factors 4.) topoisomerasesarrow_forwardBacteria are able to successfully transcribe and translate humangenes becausea. both bacteria and humans contain plasmid vectors.b. bacteria can replicate their DNA, but humans cannot.c. human and bacterial ribosomes are vastly different.d. the genetic code is nearly universal.arrow_forward
- Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: The cas genes on the bacterial genome contains a frameshift deletion mutation that alters the function of the protein The bacteria will be unable to elicit an immune response and will succumb to the phase infectionarrow_forwardYou are determined to produce an antiserum for the next pandemic that has hit the world. You have found a gene of interest in an animal in the rainforest which produces a unique protein that blocks the reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria.arrow_forwardPlasmids are important in biotechnology because they are Group of answer choices a. a vehicle for the insertion of foreign genes into bacteria b. recognition sites on recombinant DNA strands c. surfaces for protein synthesis in eukaryotic recombinants d. viruses incorporated into the host DNAarrow_forward
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