Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 19, Problem 4TYK
Summary Introduction
Introduction: Retrovirus is a widespread infectious agent that has been identified in many eukaryotes including humans. Retroviruses are of great importance as they are mostly linked with diseases such as AIDS and cancers. Retroviruses are icosahedral viruses that have an envelope. They have single-stranded RNA as genetic material.
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Proteins that bind to a specific DNA sequence and help control the recruitment of
RNA polymerase are called ...
1.) CRISPR/Cas9
2.) restriction enzymes
3.) transcription factors
4.) topoisomerases
Which of the following would be the first step in biosynthesis of a virus with a - (minus) strand of RNA?
A. Synthesis of double stranded RNA from a DNA template
B. Transcription of mRNA from DNA
C. Synthesis of DNA from an RNA template
D. Synthesis of double stranded RNA from an RNA template
E. Synthesis of DNA from a DNA template
Plasmids are important in biotechnology because they are
Group of answer choices
a. a vehicle for the insertion of foreign genes into bacteria
b. recognition sites on recombinant DNA strands
c. surfaces for protein synthesis in eukaryotic recombinants
d. viruses incorporated into the host DNA
Chapter 19 Solutions
Study Guide for Campbell Biology
Ch. 19 - In the following diagram of lytic and lysogenic...Ch. 19 - Summarize the flow of genetic information during...Ch. 19 - How does a virus spread throughout a plant? Are...Ch. 19 - Create a concept map that describes the lytic and...Ch. 19 - Prob. 2SYKCh. 19 - Prob. 1TYKCh. 19 - Prob. 2TYKCh. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - T2 and T4 are two types of bacteriophage that...
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- Which of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forward8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B. Used to test for the presence of hundreds to thousands of active genes at the same tim C. Used as a vector to replicate foreign DNA inside bacteria D. Able to cut double stranded DNA at almost any desired location for genome editing Which of the following is not an example of RNA processing. the addition of the poly-A tail to mRNA in the nucleus spliceosomes cutting mRNA in the nucleus the addition of the 3' cap to mRNA in the nucleus the joining together of exons in the nucleusarrow_forwardIn nucleotide excision repair in E. coli, the function of the UvrA/UvrB complex is toa. detect DNA damage.b. make cuts on both sides of the damage.c. remove the damaged piece of DNA.d. replace the damaged DNA with undamaged DNA.arrow_forward
- What is the function of reverse transcriptase in retroviruses? O A. It converts host cell RNA into viral DNA. B. It uses viral RNA as a template for making more viral RNA genomes. O. It translates viral RNA into proteins. D. It uses viral RNA as a template for DNA synthesis, that is, it makes complementary DNA.arrow_forwardWhich of the following is defined as transformation? A. Transfer of genetic information via a plasmid B. Virus infects a host and deposits genetic material C. Free floating DNA is uptaken D. Transfer of genetic information from one bacterium to another 5.arrow_forwardWhich of the following is a function of a restriction endonuclease? a. It cleaves a DNA molecule at a specific site. b. It cleaves a DNA molecule at random locations. c. It removes specific codons from an mRNA molecule. d. It removes specific exons from an mRNA molecule.arrow_forward
- Which of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forwardhas been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a Transgenic genome b. Recombinant DNA sequence C. Knockdown gene d. Synthetic genome e. Recombinant plasmid xarrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forward
- Which of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardBacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionarrow_forwardWhat carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forward
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