Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 19, Problem 8P
A classic way to isolate thymidylate synthase—negative mutants of bacteria is to treat a growing culture with thymidine and trimethoprim. Most of the cells are killed, and the survivors are greatly enriched in thymidylate synthase—negative mutants.
a. What
b. What is the biochemical rationale for the selection? (That is, why are the mutants not killed under these conditions?)
c. How would the procedure need to be modified to select mammalian cell mutants defective in thymidylate synthase?
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Chapter 19 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 19 - Prob. 1PCh. 19 - Prob. 2PCh. 19 - Predict the effects of the following compounds on...Ch. 19 - Prob. 4PCh. 19 - Prob. 5PCh. 19 - Prob. 6PCh. 19 - Prob. 7PCh. 19 - A classic way to isolate thymidylate...Ch. 19 - Prob. 9PCh. 19 - Prob. 10P
Ch. 19 - Prob. 11PCh. 19 - Prob. 12PCh. 19 - Prob. 13PCh. 19 - Prob. 14PCh. 19 - Prob. 15PCh. 19 - Prob. 16PCh. 19 - Prob. 17PCh. 19 - Prob. 18PCh. 19 - Prob. 19PCh. 19 - Prob. 20PCh. 19 - Prob. 21PCh. 19 - Prob. 22PCh. 19 - Prob. 23PCh. 19 - Prob. 24PCh. 19 - Prob. 25PCh. 19 - Prob. 26PCh. 19 - Prob. 27P
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