FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 19, Problem 20E
Summary Introduction
To predict: The effect of an uncoupler such as dinitrophenol on production of NADPH in a chloroplast.
Concept introduction:
An uncoupler called dinitrophenol (DNP) has the ability to separate the electrons flow and the pumping of H+ ions for the synthesis of ATP. This means that the energy acquired from the electron transfer cannot be utilized for the ATP production.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 19 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 19 - Prob. 1ECh. 19 - 2. Green sulfur bacteria use H2S as an electron...Ch. 19 - 3. The “red tide” is a massive proliferation of...Ch. 19 - 4. Explain why the chlorophylls in...Ch. 19 - Prob. 5ECh. 19 - Prob. 6ECh. 19 - Prob. 7ECh. 19 - 8. H218O is added to a suspension of chloroplasts...Ch. 19 - 9 Describe the functional similarities between the...Ch. 19 - Prob. 10E
Ch. 19 - Prob. 11ECh. 19 - Prob. 12ECh. 19 - 13. Although tile net equation for photosynthesis...Ch. 19 - 14. Why is it possible for chloroplasts to absorb...Ch. 19 - 15. Predict the effect of witling myxothiazol, an...Ch. 19 - Prob. 16ECh. 19 - Prob. 17ECh. 19 - 18. Chloroplast ATP synthase contains 14 c...Ch. 19 - 19. Predict the effect of an uncoupler such as...Ch. 19 - Prob. 20ECh. 19 - Prob. 21ECh. 19 - 22. Would the activity of the A, thaliana K+...Ch. 19 - Prob. 23ECh. 19 - Prob. 24ECh. 19 - Prob. 25ECh. 19 - 26. The shell of the cyanobacterial carboxysome...Ch. 19 - 27. In an effort to increase the efficiency of...Ch. 19 - 28. Describe the effects of an increase in oxygen...Ch. 19 - 29. The leaves of some species of desert plants...Ch. 19 - 30. Plants must obtain CO2 but avoid the loss of...Ch. 19 - 31. How might the increase in atmospheric [CO2]...Ch. 19 - Prob. 32ECh. 19 - 33. Chlorophyll f, which occurs in some species of...Ch. 19 - 34. The cyanobacterium Oscillatoria sancta appears...Ch. 19 - Prob. 35CQCh. 19 - Prob. 36CQCh. 19 - 37. Under conditions of very high light intensity,...Ch. 19 - 38. Which of the mechanisms for dissipating light...Ch. 19 - 39. Calculate the energy cost of the Calvin cycle...Ch. 19 - 40. Oil is a major energy-storage molecule in most...Ch. 19 - 41. One aspect of climate change is that average...Ch. 19 - 42. Experiments to address the day-night...Ch. 19 - 43. If a C3 plant and a C4 plant are placed...
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