FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 19, Problem 16E
Summary Introduction
To explain: The reason why knocking out a gene for a chloroplast fatty acid desaturase increases the rate of photosynthesis at 40°C.
Concept introduction:
Photosynthesis is a vital process in primary producers, plants. Plants use the light energy and the carbon dioxide (CO2) from the atmosphere and produce sugars that are stored in various parts of the plants. The enzyme fatty acid desaturase (FADs) plays an important role in the development of plants. They function to introduce double bonds into the hydrocarbon fatty acids chains in order to produce unsaturated fatty acids.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 19 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 19 - Prob. 1ECh. 19 - 2. Green sulfur bacteria use H2S as an electron...Ch. 19 - 3. The “red tide” is a massive proliferation of...Ch. 19 - 4. Explain why the chlorophylls in...Ch. 19 - Prob. 5ECh. 19 - Prob. 6ECh. 19 - Prob. 7ECh. 19 - 8. H218O is added to a suspension of chloroplasts...Ch. 19 - 9 Describe the functional similarities between the...Ch. 19 - Prob. 10E
Ch. 19 - Prob. 11ECh. 19 - Prob. 12ECh. 19 - 13. Although tile net equation for photosynthesis...Ch. 19 - 14. Why is it possible for chloroplasts to absorb...Ch. 19 - 15. Predict the effect of witling myxothiazol, an...Ch. 19 - Prob. 16ECh. 19 - Prob. 17ECh. 19 - 18. Chloroplast ATP synthase contains 14 c...Ch. 19 - 19. Predict the effect of an uncoupler such as...Ch. 19 - Prob. 20ECh. 19 - Prob. 21ECh. 19 - 22. Would the activity of the A, thaliana K+...Ch. 19 - Prob. 23ECh. 19 - Prob. 24ECh. 19 - Prob. 25ECh. 19 - 26. The shell of the cyanobacterial carboxysome...Ch. 19 - 27. In an effort to increase the efficiency of...Ch. 19 - 28. Describe the effects of an increase in oxygen...Ch. 19 - 29. The leaves of some species of desert plants...Ch. 19 - 30. Plants must obtain CO2 but avoid the loss of...Ch. 19 - 31. How might the increase in atmospheric [CO2]...Ch. 19 - Prob. 32ECh. 19 - 33. Chlorophyll f, which occurs in some species of...Ch. 19 - 34. The cyanobacterium Oscillatoria sancta appears...Ch. 19 - Prob. 35CQCh. 19 - Prob. 36CQCh. 19 - 37. Under conditions of very high light intensity,...Ch. 19 - 38. Which of the mechanisms for dissipating light...Ch. 19 - 39. Calculate the energy cost of the Calvin cycle...Ch. 19 - 40. Oil is a major energy-storage molecule in most...Ch. 19 - 41. One aspect of climate change is that average...Ch. 19 - 42. Experiments to address the day-night...Ch. 19 - 43. If a C3 plant and a C4 plant are placed...
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