The reason behind a complementary deoxyribonucleic acid (cDNA) library constructed from human brain tissue being both similar to and different from a cDNA library constructed from the human pancreatic tissue. Introduction: A collection of different cloned deoxyribonucleic acid (DNA) sequences from an organism is called a gene library. There are usually two types of gene library, namely: genomic library and complementary DNA (cDNA) library, which depends on the source of DNA used. A collection of clones containing cDNA molecules that are usually synthesized from messenger ribonucleic acid (mRNA) molecules in a cell of an organism is called cDNA library. It contains only the coding regions of a genomic material of an organism, unlike genomic library which contains the whole genome of an organism.

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O Macmillan Learning Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pKa values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 histidine 9.17 1.82 4.25 6.00 tryptophan 9.39 2.38 serine 9.15 2.21 glycine 9.60 2.34 leucine 9.60 2.36 arginine 9.04 2.17 12.48 proline 10.96 1.99 Calculate the net charge of the molecule at pH 3. net charge at pH 3: Calculate the net charge of the molecule at pH 8. net charge at pH 8: Calculate the net charge of the molecule at pH 11. net charge at pH 11: Estimate the isoelectric point (pl) for this peptide. pl:

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Chapter 18.3, Problem 4R

Summary Introduction

To review:

The reason behind a complementary deoxyribonucleic acid (cDNA) library constructed from human brain tissue being both similar to and different from a cDNA library constructed from the human pancreatic tissue.

Introduction:

A collection of different cloned deoxyribonucleic acid (DNA) sequences from an organism is called a gene library. There are usually two types of gene library, namely: genomic library and complementary DNA (cDNA) library, which depends on the source of DNA used. A collection of clones containing cDNA molecules that are usually synthesized from messenger ribonucleic acid (mRNA) molecules in a cell of an organism is called cDNA library. It contains only the coding regions of a genomic material of an organism, unlike genomic library which contains the whole genome of an organism.

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O Macmillan Learning Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pKa values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 histidine 9.17 1.82 4.25 6.00 tryptophan 9.39 2.38 serine 9.15 2.21 glycine 9.60 2.34 leucine 9.60 2.36 arginine 9.04 2.17 12.48 proline 10.96 1.99 Calculate the net charge of the molecule at pH 3. net charge at pH 3: Calculate the net charge of the molecule at pH 8. net charge at pH 8: Calculate the net charge of the molecule at pH 11. net charge at pH 11: Estimate the isoelectric point (pl) for this peptide. pl:

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