The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order: a. Transform host cells. b. Select colonies for antibiotic resistance. c. Select colonies that do not grow under ultraviolet light. d. Digest vector and foreign DNA with a restriction enzyme. e. Ligate the digested plasmid together with the foreign DNA. Introduction: Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

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Answer 1

Question

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

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Answer 2

Question

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

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Answer 3

Question

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

Expert Solution & Answer

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Answer 4

Question

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

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Answer 5

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

Answer 6

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

Answer 7

Chapter 18.2, Problem 2R

Summary Introduction

To review:

The sequential steps for inserting a foreign DNA (deoxyribonucleic acid) into a plasmid or vector by arranging the below-mentioned steps in a correct order:

a. Transform host cells.

b. Select colonies for antibiotic resistance.

c. Select colonies that do not grow under ultraviolet light.

d. Digest vector and foreign DNA with a restriction enzyme.

e. Ligate the digested plasmid together with the foreign DNA.

Introduction:

Plasmids are small, extra-chromosomal DNA molecules. They are usually circular and are relatively smaller (2000–6000 base pairs). There are often multiple restriction sites present in a plasmid sequence. Thus, this makes it easier for the insertion of a new foreign DNA. In the given case, it is assumed that a plasmid vector contains genes for green fluorescent protein (GFP) as well as for ampicillin resistance. A restriction site is present on the gene for GFP only. A foreign DNA needs to be inserted into this plasmid, which in turn, is to be inserted into the bacteria.

Answer 8

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Answer 11

Ch. 18.1 - Prob. 1R Ch. 18.1 - Prob. 2R Ch. 18.2 - Prob. 1R Ch. 18.2 - Prob. 2R Ch. 18.3 - Prob. 1R Ch. 18.3 - Prob. 2R Ch. 18.3 - Prob. 3R Ch. 18.3 - Prob. 4R Ch. 18.4 - Prob. 1R Ch. 18.4 - Prob. 2R

Solution Summary: The author describes the steps for inserting foreign DNA into a plasmid or vector.

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