EBK HUMAN PHYSIOLOGY
15th Edition
ISBN: 9781260163049
Author: Fox
Publisher: MCGRAW HILL BOOK COMPANY
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Chapter 18, Problem 26RA
Distinguish between chylomicrons, very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 18 Solutions
EBK HUMAN PHYSIOLOGY
Ch. 18 - Prob. 1aCPCh. 18 - Describe the structure and function of the mucosa,...Ch. 18 - Prob. 2bCPCh. 18 - Describe the structure and function of the lower...Ch. 18 - Prob. 3bCPCh. 18 - Prob. 4aCPCh. 18 - Explain how peptic ulcers are produced and why...Ch. 18 - Explain how gastrin and vagus nerve stimulation...Ch. 18 - Describe the adaptations of the small intestine...Ch. 18 - Prob. 6CP
Ch. 18 - Prob. 7CPCh. 18 - Prob. 8aCPCh. 18 - Prob. 8bCPCh. 18 - Prob. 9CPCh. 18 - Prob. 10aCPCh. 18 - Prob. 10bCPCh. 18 - Prob. 10cCPCh. 18 - Prob. 11aCPCh. 18 - Prob. 11bCPCh. 18 - Prob. 12CPCh. 18 - Prob. 13aCPCh. 18 - Describe the mechanisms involved in the intestinal...Ch. 18 - Prob. 14CPCh. 18 - Prob. 15CPCh. 18 - Prob. 16aCPCh. 18 - List each enzyme involved in protein digestion....Ch. 18 - Describe how bile aids both the digestion and...Ch. 18 - Trace the pathway and fate of a molecule of...Ch. 18 - Prob. 17cCPCh. 18 - Which of these statements about intrinsic factor...Ch. 18 - Prob. 2RACh. 18 - Which of these statements about gastric secretion...Ch. 18 - Prob. 4RACh. 18 - Which of these statements about trypsin is...Ch. 18 - Prob. 6RACh. 18 - Prob. 7RACh. 18 - The first organ to receive the blood-borne...Ch. 18 - Prob. 9RACh. 18 - Prob. 10RACh. 18 - Prob. 11RACh. 18 - Which of these statements about vitamin is...Ch. 18 - Which of these statements about starch digestion...Ch. 18 - Prob. 14RACh. 18 - Which of these statements about contraction of...Ch. 18 - Prob. 16RACh. 18 - Prob. 17RACh. 18 - Which of the following statements about the liver...Ch. 18 - Prob. 19RACh. 18 - Prob. 20RACh. 18 - Prob. 21RACh. 18 - Describe the mechanisms that are believed to...Ch. 18 - Prob. 23RACh. 18 - Explain how jaundice is produced when (a) the...Ch. 18 - Prob. 25RACh. 18 - Distinguish between chylomicrons, very-low-density...Ch. 18 - Prob. 27RACh. 18 - Trace the course of blood flow through the liver...Ch. 18 - Prob. 29RACh. 18 - Describe the reflexes controlling the stomach,...Ch. 18 - Prob. 31RACh. 18 - Prob. 32RACh. 18 - Prob. 33RACh. 18 - Prob. 34RACh. 18 - Prob. 35RACh. 18 - Prob. 36RACh. 18 - Prob. 37RACh. 18 - Prob. 38RACh. 18 - Prob. 39RACh. 18 - Prob. 40RACh. 18 - Refer to figure 18. I 4 to answer the following...Ch. 18 - Prob. 42RACh. 18 - Prob. 43RA
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