NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Chapter 18, Problem 1CT
Summary Introduction
To review:
To confirm a positive ELISA (enzyme-linked immunosorbent assay) for HIV (human immunodeficiency virus) test is confirmed using western blotting and notanother way around.
Introduction:
HIV is a virus that can affect the human body and leads to the condition known as AIDS (acquired immunodeficiency syndrome). This disease basically affects the immune system of the individual and makes them prone to different infectious diseases or illness. AIDS can be detected by the technique known as ELISA.
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Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?
You performed an Elisa test in the lab to detect antibody against HIV in the patient's serum. The test was found to be 96% sensitive. What does that mean?
A western blot was used on the same sample to confirm, and the test was found to be 99% specific. What does that mean?
why can't we use western blotting test as diagnostic test for hiv alone without using ELISA?
Chapter 18 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 18 - How is immune globulin different from hyperimmune...Ch. 18 - Describe two advantages of an attenuated vaccine...Ch. 18 -
3. Describe two advantages of an inactivated...Ch. 18 - Prob. 4SACh. 18 - Prob. 5SACh. 18 - Prob. 6SACh. 18 - Prob. 7SACh. 18 - What is an advantage of the fluorescent antibody...Ch. 18 - Is blood typing an example of a precipitation...Ch. 18 - Prob. 10SA
Ch. 18 - Prob. 1MCCh. 18 - Prob. 2MCCh. 18 - Prob. 3MCCh. 18 - Prob. 4MCCh. 18 - Prob. 5MCCh. 18 - Haemophilus influenzae is an encapsulated...Ch. 18 -
7. In quantifying antibodies in a patient's...Ch. 18 - Prob. 8MCCh. 18 - Prob. 9MCCh. 18 - Prob. 10MCCh. 18 - A new parent asks you which vaccines the CDC...Ch. 18 - Prob. 2ACh. 18 - Prob. 1CTCh. 18 - Prob. 2CT
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Similar questions
- Why is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure is used only as the confirmation test?arrow_forwardThe substrate For the secondary antibody used in the lab for western blothing is : TMB TritonX-100 tween BCIP/NBTarrow_forwardEIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure usedto confirm screening tests that are positive for humanimmunodeficiency virus (HIV)?arrow_forward
- To detect the protein in Western blotting, antibody is used antigen is used DNA probe is used RNA probe is usedarrow_forwardWhat is a recombinant DNA vaccine? List two such vaccines. State their advantages.arrow_forwardWhat is the effect of not adding the primary antibody to the ELISA reaction?arrow_forward
- which combination of tests would be the most reliable and practical in diagonsing an HIV infection? a)Elisa, Oraquick adance b)P 24 antiboy, Western blot c)Bombo Antigen/antibody quick test, PCR testing d)oraquick advance antibody test, ELISA e) Western blot, PCR testingarrow_forwardIn this diagram, why is anti-human IgG used in the indirect test but not in the direct test?arrow_forwardIf you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.arrow_forward
- You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asaparrow_forwardWhy do vaccine manufacturers begin with tests on animals or cell lines before moving on to adult human subjects?arrow_forwardSouthern blotting technique is used ina) Monoclonal antibody productionb) In vitro culturec) Genetic finger printingd) Polymerase chain reactionarrow_forward
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