NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Chapter 18, Problem 1CT
Summary Introduction

To review:

To confirm a positive ELISA (enzyme-linked immunosorbent assay) for HIV (human immunodeficiency virus) test is confirmed using western blotting and notanother way around.

Introduction:

HIV is a virus that can affect the human body and leads to the condition known as AIDS (acquired immunodeficiency syndrome). This disease basically affects the immune system of the individual and makes them prone to different infectious diseases or illness. AIDS can be detected by the technique known as ELISA.

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Students have asked these similar questions
Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive).  In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus.  In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture.  Why do you think this might be necessary?
You performed an Elisa test in the lab to detect antibody against HIV in the patient's serum. The test was found to be 96% sensitive. What does that mean? A western blot was used on the same sample to confirm, and the test was found to be 99% specific. What does that mean?
why can't we use western blotting test as diagnostic test for hiv alone without using ELISA?
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