All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure. Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Question

Want to see the full answer?

Answer 1

Question

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Three of the many recessive mutations in Drosophila melanogaster that affect body color, wing shape, or bristle morphology are black (b) body versus grey in wild type, dumpy (dp), obliquely truncated wings versus long wings in the male, and hooked (hk) bristles versus not hooked in the wild type. From a cross of a dumpy female with a black and hooked male, all of the F1 were wild type for all three of the characters. The testcross of an F1 female with a dumpy, black, hooked male gave the following results: Trait Number of individuals Wild type 169 Black 19 Black, hooked 301 Dumpy, hooked 21 Hooked, dumpy, black 172 Dumpy, black 6 Dumpy 305 Hooked 8 Determine the order of the genes and the mapping distance between genes. Determine the coefficient of confidence for the portion of the chromosome involved in the cross. How much interference takes place in the cross?

What happens to a microbes membrane at colder temperature?

Genes at loci f, m, and w are linked, but their order is unknown. The F1 heterozygotes from a cross of FFMMWW x ffmmww are test crossed. The most frequent phenotypes in the test cross progeny will be FMW and fmw regardless of what the gene order turns out to be. What classes of testcross progeny (phenotypes) would be least frequent if locus m is in the middle? What classes would be least frequent if locus f is in the middle? What classes would be least frequent if locus w is in the middle?

Answer 2

Question

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 6

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 7

Chapter 18, Problem 14Q

A.

Summary Introduction

To explain: All cells including all their phases in their cell cycle are expected to contain radioactive DNA after labeling procedure.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 8

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 9

B.

Summary Introduction

To explain: The reason why initially there were no mitotic cells that possessed radioactive DNA.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 10

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 11

C.

Summary Introduction

To explain: The rise and fall and again the rise of the curve.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 12

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 13

D.

Summary Introduction

To estimate: The length of G2 phase from the graph obtained.

Concept introduction: Cells are cultured in culture mediums that contain all the necessary nutrients for the cell to grow and divide. Cells that are tested for cell cycle are usually loaded with nucleotides to assess the S phase of the experiment. The DNA is usually found as chromatin reticulum, fine thread-like structures in the nucleus during interphase. When the cell is about to divide, the chromosome undergoes condensation to compact its structure. This structure when stained with suitable dyes is visible during the M phase of the cell cycle. Autoradiography is a technique where the nucleotides are radiolabelled with radioactive isotope of an atom present in them, mostly nitrogen or carbon isotope. These radiolabelled nucleotides when they are incorporated into the DNA during cell division emit radiation. The radioactivity is captured using photographic emulsion. This photographic emulsion when placed over the cells, the radioactive isotope activates the emulsion and wherever the radioactivity is exhibited black dots are observed in the emulsion.

Answer 14

Expert Solution & Answer

Answer 15

Expert Solution & Answer

Answer 16

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 17

Ch. 18 - Prob. 1Q Ch. 18 - Prob. 2Q Ch. 18 - Prob. 3Q Ch. 18 - Prob. 4Q Ch. 18 - Prob. 5Q Ch. 18 - Prob. 6Q Ch. 18 - Prob. 7Q Ch. 18 - Prob. 8Q Ch. 18 - Prob. 9Q Ch. 18 - Prob. 10Q

Concept explainers

Videos

Want to see the full answer?

Chapter 18 Solutions