EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 18, Problem 12MC
Summary Introduction
Introduction:
Human immune virus (HIV) specifically targets and destroys the T-cells and causes AIDS. The HIV attaches specifically through the CD4 receptors present on the T-cells, and gains the entry into cells, where it replicates and stays as a latent virus. It gets integrated with the cell’s genome and it is passed to all the daughter cells.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 18 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 18 - Prob. 1CCSCh. 18 - Prob. 1TMWCh. 18 - Prob. 2TMWCh. 18 - A Case of AIDS A 25-year-old man is admitted to...Ch. 18 - Prob. 3TMWCh. 18 - The immunoglobulin class that mediates type I...Ch. 18 - The major inflammatory mediator released by...Ch. 18 - Hemolytic disease of the new born is caused by...Ch. 18 - Farmers lung is a hypersensitivity pneumonitis...Ch. 18 - A positive tuberculin skin test indicates that a...
Ch. 18 - Which of the following is an autoimmune disease?...Ch. 18 - When a surgeon conducts a cardiac bypass operation...Ch. 18 - A deficiency of both B cells and T cells is most...Ch. 18 - Prob. 9MCCh. 18 - What do medical personnel administer to counteract...Ch. 18 - Which of the following is not typically part of an...Ch. 18 - Prob. 12MCCh. 18 - Prob. 13MCCh. 18 - Prob. 14MCCh. 18 - Prob. 15MCCh. 18 - Prob. 1MTFCh. 18 - Prob. 2MTFCh. 18 - Prob. 3MTFCh. 18 - Prob. 4MTFCh. 18 - Modified True/False 5. ___ Graft-versus-host...Ch. 18 - Prob. 1MCh. 18 - Prob. 1SACh. 18 - Prob. 2SACh. 18 - Prob. 3SACh. 18 - Contrast autografts, isografts, allografts, and...Ch. 18 - Prob. 5SACh. 18 - Prob. 1VICh. 18 - Prob. 2VICh. 18 - Prob. 3VICh. 18 - Prob. 1CTCh. 18 - Why cant physicians use skin tests similar to the...Ch. 18 - Prob. 3CTCh. 18 - Prob. 4CTCh. 18 - What types of illnesses cause death in patients...Ch. 18 - Prob. 6CTCh. 18 - Why do the blisters of positive tuberculin...Ch. 18 - Retroviruses such as HIV use RNA as a primer for...Ch. 18 - Prob. 9CTCh. 18 - Reverse transcriptase is notoriously sloppy in...Ch. 18 - A patient arrives at the doctors office with a...Ch. 18 - Prob. 12CTCh. 18 - Prob. 13CTCh. 18 - Prob. 1CM
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