CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
8th Edition
ISBN: 9781259880193
Author: SALADIN
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 17.4, Problem 12AYLO
Three kinds of interactions that can occur when two or more of them act simultaneously on a target cell
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1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Chapter 17 Solutions
CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
Ch. 17.1 - Define the word hormone and distinguish a hormone...Ch. 17.1 - Prob. 2BYGOCh. 17.1 - Prob. 3BYGOCh. 17.1 - Prob. 4BYGOCh. 17.1 - Discuss why the target-cell concept is essential...Ch. 17.1 - The importance of intercellular communication for...Ch. 17.1 - The general term for the cells and glands that...Ch. 17.1 - Prob. 3AYLOCh. 17.1 - Prob. 4AYLOCh. 17.1 - Prob. 5AYLO
Ch. 17.2 - Prob. 6BYGOCh. 17.2 - Prob. 7BYGOCh. 17.2 - Prob. 8BYGOCh. 17.2 - In what sense does the pituitary take orders from...Ch. 17.2 - Prob. 10BYGOCh. 17.2 - Prob. 1AYLOCh. 17.2 - Prob. 2AYLOCh. 17.2 - Prob. 3AYLOCh. 17.2 - Two hormones synthesized in the hypothalamus and...Ch. 17.2 - Prob. 5AYLOCh. 17.2 - Two hormones secreted by the posterior pituitary,...Ch. 17.2 - Prob. 7AYLOCh. 17.2 - Prob. 8AYLOCh. 17.3 - Prob. 11BYGOCh. 17.3 - Prob. 12BYGOCh. 17.3 - Prob. 13BYGOCh. 17.3 - Prob. 14BYGOCh. 17.3 - Prob. 15BYGOCh. 17.3 - Prob. 16BYGOCh. 17.3 - Prob. 1AYLOCh. 17.3 - Prob. 2AYLOCh. 17.3 - Prob. 3AYLOCh. 17.3 - Anatomy of the parathyroid glands; their hormone...Ch. 17.3 - Prob. 5AYLOCh. 17.3 - Prob. 6AYLOCh. 17.3 - Three tissue zones of the adrenal cortex, the...Ch. 17.3 - Prob. 8AYLOCh. 17.3 - Prob. 9AYLOCh. 17.3 - Hormones produced by the following tissues and...Ch. 17.4 - What are the three chemical classes of hormones?...Ch. 17.4 - Prob. 18BYGOCh. 17.4 - Prob. 19BYGOCh. 17.4 - Prob. 20BYGOCh. 17.4 - Prob. 21BYGOCh. 17.4 - Prob. 1AYLOCh. 17.4 - Prob. 2AYLOCh. 17.4 - Prob. 3AYLOCh. 17.4 - Prob. 4AYLOCh. 17.4 - The Types of stimuli that elicit hormone...Ch. 17.4 - Thyroid hormone synthesis and secretionCh. 17.4 - Prob. 7AYLOCh. 17.4 - Prob. 8AYLOCh. 17.4 - Which hormones require second messengers to...Ch. 17.4 - How signal amplification enables small amounts of...Ch. 17.4 - How target cells modulate their hormone...Ch. 17.4 - Three kinds of interactions that can occur when...Ch. 17.4 - Prob. 13AYLOCh. 17.5 - Define stress from the standpoint of...Ch. 17.5 - Describe the stages of the general adaptation...Ch. 17.5 - Prob. 24BYGOCh. 17.5 - Prob. 1AYLOCh. 17.5 - Prob. 2AYLOCh. 17.5 - The three stages of the stress response; the...Ch. 17.6 - Prob. 25BYGOCh. 17.6 - Prob. 26BYGOCh. 17.6 - Prob. 27BYGOCh. 17.6 - Paracrine and autocrine secretions, examples, and...Ch. 17.6 - The general structure and metabolic precursor of...Ch. 17.6 - Prob. 3AYLOCh. 17.6 - Prob. 4AYLOCh. 17.6 - Prob. 5AYLOCh. 17.7 - Prob. 28BYGOCh. 17.7 - Prob. 29BYGOCh. 17.7 - Prob. 30BYGOCh. 17.7 - Prob. 1AYLOCh. 17.7 - Myxedema, endemic goiter, and toxic goiterCh. 17.7 - Effects of hypo- and hyperparathyroidismCh. 17.7 - Cushing syndrome and adrenogenital syndromeCh. 17.7 - Prob. 5AYLOCh. 17.7 - Prob. 6AYLOCh. 17.7 - Prob. 7AYLOCh. 17.7 - Consequences of inadequately treated DM and why...Ch. 17 - CRH secretion would not raise the blood...Ch. 17 - Prob. 2TYRCh. 17 - Prob. 3TYRCh. 17 - Prob. 4TYRCh. 17 - Prob. 5TYRCh. 17 - Prob. 6TYRCh. 17 - Prob. 7TYRCh. 17 - Prob. 8TYRCh. 17 - Prob. 9TYRCh. 17 - Prostaglandins are derived from a. phospholipase....Ch. 17 - Prob. 11TYRCh. 17 - Prob. 12TYRCh. 17 - Growth hormone hypersecretion in adulthood causes...Ch. 17 - Prob. 14TYRCh. 17 - Prob. 15TYRCh. 17 - Prob. 16TYRCh. 17 - Target cells can reduce pituitary secretion by a...Ch. 17 - Prob. 18TYRCh. 17 - Prob. 19TYRCh. 17 - ______ is a process in which a cell increases its...Ch. 17 - adeno-Ch. 17 - Prob. 2BYMVCh. 17 - Prob. 3BYMVCh. 17 - Prob. 4BYMVCh. 17 - Prob. 5BYMVCh. 17 - Prob. 6BYMVCh. 17 - Prob. 7BYMVCh. 17 - Prob. 8BYMVCh. 17 - Prob. 9BYMVCh. 17 - Prob. 10BYMVCh. 17 - Castration would lower a mans blood gonadotropin...Ch. 17 - Prob. 2WWTSCh. 17 - Prob. 3WWTSCh. 17 - Prob. 4WWTSCh. 17 - Prob. 5WWTSCh. 17 - The great majority of cases of diabetes mellitus...Ch. 17 - Prob. 7WWTSCh. 17 - A deficiency of dietary iodine would lead to...Ch. 17 - Prob. 9WWTSCh. 17 - Prob. 10WWTSCh. 17 - Prob. 1TYCCh. 17 - Suppose you were browsing in a health-food store...Ch. 17 - Prob. 3TYCCh. 17 - Prob. 4TYCCh. 17 - A young man is involved in a motorcycle accident...
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