Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 17, Problem 6P
Summary Introduction

a.

To determine:

The way in which one can identify or spot the enhancer in fly lines in which transgene has been integrated.

Introduction:

The technique of enhancer trapping was first developed by the scientists in Drosophila. The goal of this technique is to find the enhancer inside the genome of a particular cell.

Summary Introduction

b.

To determine:

The ways by which the technique of enhancer trapping can be used for the identification of expression of genes in the wings of a fly.

Introduction:

The technique of enhancer trapping is also used for the identification of active genes in the cell. In this technique, a large number of fly lines are created which possess a single copy of transgene.

Summary Introduction

c.

To determine:

Whether the homozygotes for any insertion of transgene possess a mutant phenotype.

Introduction:

The transgene was integrated inside the genome at random locations with the help of P-element mediated transfer of genes.

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY