a. To determine: The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein). Introduction: The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

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Answer 1

Question

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Answer 2

Question

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 3

Question

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 4

Question

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 5

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 6

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Answer 7

Chapter 17, Problem 15P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Answer 8

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Answer 9

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Answer 10

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Answer 11

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Answer 12

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 13

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 17 - Match each of the terms in the left column to the...Ch. 17 - Mice are usually gray, but a mouse geneticist has...Ch. 17 - Sometimes, genes transferred into the mouse genome...Ch. 17 - In mice, a group of so-called Hox genes encode...Ch. 17 - The fly eyes shown in Fig. 17.6 p. 580 are...Ch. 17 - Prob. 6P Ch. 17 - Fish and other organisms that live in the Arctic...Ch. 17 - Prob. 8P Ch. 17 - The first page of this chapter p. 575 shows a...Ch. 17 - The goal of the Knockout Mouse Project is to...

Solution Summary: The author describes gene trapping as a high-throughput approach that is utilized for the insertional type of mutations across the genome of mammals.

Concept explainers

Videos

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Want to see the full answer?

Chapter 17 Solutions

Solution Summary: The author describes gene trapping as a high-throughput approach that is utilized for the insertional type of mutations across the genome of mammals.

Concept explainers

Videos

b. To determine: Whether the genome of all mice should be integrated in a way which is described in the previous part. Introduction: A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

c. To determine: The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques. Introduction: The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Want to see the full answer?

Chapter 17 Solutions

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.