Chapter 17, Problem 54P

Determine the amino acid sequence of a polypeptide from the following data: Complete hydrolysis of the peptide yields Ala, Arg, Gly, 2 Lys, Met, Phe, Pro, 2 Ser, Tyr, and Val. Treatment with Edman’s reagent releases PTH-Val.

Carboxypeptidase A releases Ala.

Treatment with cyanogen bromide yields the following two peptides:

1. 1. Ala, 2 Lys, Phe, Pro, Ser, Tyr
2. 2. Arg, Gly, Met, Ser, Val

Treatment with trypsin yields the following three peptides:

1. 1. Gly, Lys, Met, Tyr
2. 2. Ala, Lys, Phe, Pro, Ser
3. 3. Arg, Ser, Val

Treatment with chymotrypsin yields the following three peptides:

1. 1. 2 Lys, Phe, Pro
2. 2. Arg, Gly, Met, Ser, Tyr, Val
3. 3. Ala, Ser