(a) Interpretation: The effect of arsenite the loss of citrate should be determined. Concept introduction: The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

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Answer 1

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Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

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Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

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Answer 3

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Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

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Answer 4

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Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

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Answer 5

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Answer 6

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Answer 7

Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 8

Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 9

Chapter 17, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The effect of arsenite the loss of citrate should be determined.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 10

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 11

Interpretation Introduction

(b)

Interpretation:

Explanation for the modified action of arsenite due to the addition of more citrate.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 12

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 13

Interpretation Introduction

(c)

Interpretation:

Given data proposes about the place of action of arsenite.

Concept introduction:

The citric acid cycle is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Oxaloacetate is an intermediate of the Krebs cycle which binds to the acetyl-CoA in the synthesis of citrate. Arsenite is a chemical compound that contains an arsenic oxo-anion in which the arsenic has oxidation state +3.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 17 - Prob. 1P Ch. 17 - Prob. 2P Ch. 17 - Prob. 3P Ch. 17 - Prob. 4P Ch. 17 - Prob. 5P Ch. 17 - Prob. 6P Ch. 17 - Prob. 7P Ch. 17 - Prob. 8P Ch. 17 - Prob. 9P Ch. 17 - Prob. 10P

Solution Summary: The author explains that the effect of arsenite on the loss of citrate should be determined.

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