Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 17, Problem 40P
Interpretation Introduction

(a)

Interpretation:

The amount of O2 absorbed by complete oxidation of added citrate is to be calculated.

Concept introduction:

The activity of the citric acid cycle can be observed by accounting the amount of O2. H. Krebs conducts the experiment on pigeon-breast muscle to investigate that the rate of citric acid cycle grows with the increased rate of O2 consumption.

The citric acid cycle is the third step of the biochemical energy production process. The cycle consists of the reactions from which the acetyl part of acetyl CoA is oxidized to form carbon dioxide and CoA-SH.

Interpretation Introduction

(b)

Interpretation:

On the basis of the result in part (a), consumption of oxygen in the presence of citrate is to be explained.

Concept introduction:

The activity of citric acid cycle can be observed by accounting the amount of O2. H. Krebs conducts the experiment on pigeon-breast muscle to investigate that rate of citric acid cycle increases with the increased rate of O2 consumption.

The balanced reaction of complete oxidation of citrate is,

C6H8O7+4.5O26CO2+4H2O

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism  of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
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