Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
Question
Book Icon
Chapter 17, Problem 40P
Interpretation Introduction

(a)

Interpretation:

The amount of O2 absorbed by complete oxidation of added citrate is to be calculated.

Concept introduction:

The activity of the citric acid cycle can be observed by accounting the amount of O2. H. Krebs conducts the experiment on pigeon-breast muscle to investigate that the rate of citric acid cycle grows with the increased rate of O2 consumption.

The citric acid cycle is the third step of the biochemical energy production process. The cycle consists of the reactions from which the acetyl part of acetyl CoA is oxidized to form carbon dioxide and CoA-SH.

Interpretation Introduction

(b)

Interpretation:

On the basis of the result in part (a), consumption of oxygen in the presence of citrate is to be explained.

Concept introduction:

The activity of citric acid cycle can be observed by accounting the amount of O2. H. Krebs conducts the experiment on pigeon-breast muscle to investigate that rate of citric acid cycle increases with the increased rate of O2 consumption.

The balanced reaction of complete oxidation of citrate is,

C6H8O7+4.5O26CO2+4H2O

Blurred answer
Students have asked these similar questions
carbons in each of the structures below. For instance, the central carbon of chloromethylbutane (pictured 3. A chiral carbon is a carbon that is single-bonded to four different types of groups. Identify the chiral above) is a chiral carbon. (Can you see how the groups attached to it are all chemically different?) In each of the chiral molecules below, identify all the carbons that are chiral carbons by drawing a circle around each one of them. (a) the carbohydrate glucose H O (b) the carbohydrate fructose CH₂OH 1C H-C-OH 3 HO-C-H 4 H-C-OH 5 H-C-OH 6CH₂OH D-Glucose (linear form) (c) the amino acid leucine O O H3C. HO H H- -OH CH 3 NH2 H- -OH CH₂OH OH
We always include controls in the Annexin-V-GFP/Propidium Iodide flow cytometric assay to study apoptosis. List four types of controls in this assay.  Why do we need these controls? Explain your answers.  After the flow assay, if we like to examine the morphology of the viable, early apoptotic and late apoptotic cells by confocal microscopy, what can we do and what are the expected results?
3. (2 points) Your lab partner accidentally used a pen instead of a pencil to mark the baseline and label the lanes of their TLC plate. Briefly (1-2 sentences for each point) describe (a) what would happen to the ink when you develop the TLC plate; and (b) how this would affect the experiment. 1
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
  • Text book image
    Biochemistry
    Biochemistry
    ISBN:9781305577206
    Author:Reginald H. Garrett, Charles M. Grisham
    Publisher:Cengage Learning
    Text book image
    Biology 2e
    Biology
    ISBN:9781947172517
    Author:Matthew Douglas, Jung Choi, Mary Ann Clark
    Publisher:OpenStax
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax