Biology
Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 17, Problem 3U
Summary Introduction

Introduction:

PCR stands for Polymerase chain reaction. It is a technique that is used to amplify a single fragment of DNA to generate large copies of that particular fragment of DNA. This technique was developed by Kary Mullis in 1983.

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If a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles? a. About 100 b. About 1000 c. About 10,000 d. About 1010
Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting.  c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.
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