Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 17, Problem 17.4.9AYLO
Summary Introduction
To determine:
The reason due to which high light sensitivity and high resolution cannot be obtained with a single retinal circuit. Also, explain the difference between the rod and cone circuit.
Introduction:
The photoreceptor cell includes all cells that absorb light and generate chemical signals. There are three types of cells rod, cone, and some ganglion cells. Rod and cone cells are responsible for visual images.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 17 Solutions
Human Anatomy
Ch. 17.1 - Prob. 1AWYKCh. 17.1 - Distinguish between general and special senses.Ch. 17.1 - Three schemes of receptor classification were...Ch. 17.1 - Prob. 3BYGOCh. 17.1 - Prob. 4BYGOCh. 17.1 - Prob. 5BYGOCh. 17.1 - Prob. 6BYGOCh. 17.2 - Prob. 1AWYKCh. 17.2 - What is the difference between a lingual papilla...Ch. 17.2 - Prob. 8BYGO
Ch. 17.2 - What part of an olfactory cell bears the binding...Ch. 17.2 - Prob. 10BYGOCh. 17.3 - Prob. 1AWYKCh. 17.3 - What is the benefit of having three auditory...Ch. 17.3 - Prob. 12BYGOCh. 17.3 - How does the brain recognize the difference...Ch. 17.3 - Prob. 14BYGOCh. 17.3 - Prob. 15BYGOCh. 17.4 - Prob. 1AWYKCh. 17.4 - Prob. 2AWYKCh. 17.4 - Prob. 3AWYKCh. 17.4 - Prob. 16BYGOCh. 17.4 - List as many structural and functional differences...Ch. 17.4 - Prob. 18BYGOCh. 17.4 - Prob. 19BYGOCh. 17.5 - Describe the contributions of the first pharyngeal...Ch. 17.5 - Prob. 21BYGOCh. 17.5 - Prob. 22BYGOCh. 17.5 - Prob. 23BYGOCh. 17 - The meaning of sensory receptor and the range of...Ch. 17 - Prob. 17.1.2AYLOCh. 17 - Prob. 17.1.3AYLOCh. 17 - Prob. 17.1.4AYLOCh. 17 - Prob. 17.1.5AYLOCh. 17 - The types of sensory nerve endings considered to...Ch. 17 - Prob. 17.1.7AYLOCh. 17 - Prob. 17.1.8AYLOCh. 17 - Prob. 17.1.9AYLOCh. 17 - Prob. 17.1.10AYLOCh. 17 - Prob. 17.1.11AYLOCh. 17 - Prob. 17.1.12AYLOCh. 17 - Prob. 17.1.13AYLOCh. 17 - The relationship of taste buds to the lingual...Ch. 17 - Prob. 17.2.2AYLOCh. 17 - Prob. 17.2.3AYLOCh. 17 - Prob. 17.2.4AYLOCh. 17 - Prob. 17.2.5AYLOCh. 17 - Prob. 17.2.6AYLOCh. 17 - Prob. 17.2.7AYLOCh. 17 - Prob. 17.3.1AYLOCh. 17 - Prob. 17.3.2AYLOCh. 17 - The parts of the middle ear, including its three...Ch. 17 - Prob. 17.3.4AYLOCh. 17 - The anatomy of the cochlea and the functional...Ch. 17 - Prob. 17.3.6AYLOCh. 17 - How cochlear function enables the brain to...Ch. 17 - Prob. 17.3.8AYLOCh. 17 - The differences between static and dynamic...Ch. 17 - Prob. 17.3.10AYLOCh. 17 - The action of the otolithic membrane in...Ch. 17 - Prob. 17.3.12AYLOCh. 17 - Prob. 17.3.13AYLOCh. 17 - The projection pathways taken by signals of...Ch. 17 - Prob. 17.4.1AYLOCh. 17 - Prob. 17.4.2AYLOCh. 17 - Prob. 17.4.3AYLOCh. 17 - Prob. 17.4.4AYLOCh. 17 - Prob. 17.4.5AYLOCh. 17 - Prob. 17.4.6AYLOCh. 17 - Prob. 17.4.7AYLOCh. 17 - Prob. 17.4.8AYLOCh. 17 - Prob. 17.4.9AYLOCh. 17 - The projection pathways taken by retinal signals...Ch. 17 - Prob. 17.4.11AYLOCh. 17 - Prob. 17.5.1AYLOCh. 17 - Prob. 17.5.2AYLOCh. 17 - Prob. 17.5.3AYLOCh. 17 - How the lens, vitreous body, anterior chamber,...Ch. 17 - Hot and cold stimuli are detected by free nerve...Ch. 17 - Prob. 2TYRCh. 17 - Prob. 3TYRCh. 17 - Prob. 4TYRCh. 17 - The sensory neurons that begin in the spiral organ...Ch. 17 - The spiral organ rests on the tympanic membrane....Ch. 17 - Prob. 7TYRCh. 17 - Prob. 8TYRCh. 17 - Prob. 9TYRCh. 17 - Prob. 10TYRCh. 17 - The most finely detailed vision occurs when an...Ch. 17 - Fibers of the optic nerve come from the...Ch. 17 - A sensory nerve ending specialized to detect...Ch. 17 - The gelatinous membranes of the macula sacculi and...Ch. 17 - Three rows of ____________ in the cochlea have...Ch. 17 - The __________ is a tiny bone that vibrates in the...Ch. 17 - The ___________ of the midbrain receive auditory...Ch. 17 - The apical microvilli of a gustatory cell are...Ch. 17 - Olfactory neurons synapse with mitral cells and...Ch. 17 - Prob. 20TYRCh. 17 - State a meaning of each word element and give a...Ch. 17 - State a meaning of each word element and give a...Ch. 17 - State a meaning of each word element and give a...Ch. 17 - State a meaning of each word element and give a...Ch. 17 - State a meaning of each word element and give a...Ch. 17 - State a meaning of each word element and give a...Ch. 17 - State a meaning of each word element and give a...Ch. 17 - Prob. 8BYMVCh. 17 - State a meaning of each word element and give a...Ch. 17 - Prob. 10BYMVCh. 17 - Determine which five of the following statements...Ch. 17 - Briefly explain why each of the following...Ch. 17 - Prob. 3TOFCh. 17 - Briefly explain why each of the following...Ch. 17 - Determine which five of the following statements...Ch. 17 - Prob. 6TOFCh. 17 - Prob. 7TOFCh. 17 - Prob. 8TOFCh. 17 - Briefly explain why each of the following...Ch. 17 - Briefly explain why each of the following...Ch. 17 - Prob. 1TYCCh. 17 - What type of cutaneous receptor enables you to...Ch. 17 - Predict the consequences of a hypothetical...Ch. 17 - Prob. 4TYCCh. 17 - Prob. 5TYC
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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