The overall charge of a polar neutral amino acid at a pH above its pI. Concept introduction: ➢ Amino acid: Any molecule contains both amino and acid group Ex: ➢ The R − group is polar ( − CH 2 − OH, − CH 2 − S − CH 3 etc ) then called polar amino acid. ➢ The amino acid has two groups One acts base and other acts as acid. So net charge or form of amino acid depends upon pH value ➢ Amino acid is any molecule that contains both amino and acid group. If pH = pI net charge on amino acid is zero called zwitterion form contains equal no of + ve and − ve charge. ➢ If pH > pI , then at this pH H 3 N + − ➢ acts as acid and donates one proton ( H + ) ➢ to form − NH 2 And it has total charge = 1 − ➢ If pH < pI , then Acts as base and accept one proton form solution and convert to . So at such pH it has total charge = 1 +

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Chapter 16.2, Problem 16.12QAP

Interpretation Introduction

Interpretation: The overall charge of a polar neutral amino acid at a pH above its pI.

Concept introduction:

➢ Amino acid: Any molecule contains both amino and acid group

Ex:

➢ The $R -$ group is polar $(- {CH}_{2} - OH, - {CH}_{2} - S - {CH}_{3} etc)$ then called polar amino acid.

➢ The amino acid has two groups

One acts base and other acts as acid. So net charge or form of amino acid depends upon $pH value$

➢ Amino acid is any molecule that contains both amino and acid group. If $pH = pI$ net charge on amino acid is zero called zwitterion form contains equal no of $+ ve and - ve$ charge.

➢ If $pH > pI$ , then at this ${pH H}_{3} N^{+} -$

➢ acts as acid and donates one proton $(H^{+})$

➢ to form $- {NH}_{2}$

And it has total charge $= 1 -$

➢ If $pH < pI$ , then

Acts as base and accept one proton form solution and convert to

. So at such $pH$

it has total charge $= 1 +$

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