(a) Interpretation: Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK). Concept introduction: Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

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Answer 1

Question

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

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Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa). Lane 1: buffer Lane 2 : Ladder Lane 3: Lysate Lane 4: Flowthrough Lane 5: Wash Lanes 6-8: E1, E2, E3 Lane 9: Dialyzed VRK1 Lane 10: LDH

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You have isolated a protein and determined that the native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. Describe the architecture of the polypeptide component of this enzyme.

Answer 2

Question

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

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Answer 3

Question

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

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Answer 4

Question

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

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Answer 5

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 6

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 7

Chapter 16, Problem 44P

Interpretation Introduction

(a)

Interpretation:

Effect on glycolysis on the loss of the allosteric site of ATP in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 8

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 9

Interpretation Introduction

(b)

Interpretation:

Effect on glycolysis on the loss of binding site of citrate in phosphofructokinase (PFK).

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 10

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 11

Interpretation Introduction

(c)

Interpretation:

Effect on the rate of glycolysis on the loss of phosphatase domain of bifunctional enzyme that controls the level of fructose-2,6-bisphosphate.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 12

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 13

Interpretation Introduction

(d)

Interpretation:

Effect on glycolysis rate, if there is loss of binding site for fructose-1,6-bisphosphate on pyruvate kinase enzyme.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 16 - Prob. 1P Ch. 16 - Prob. 2P Ch. 16 - Prob. 3P Ch. 16 - Prob. 4P Ch. 16 - Prob. 5P Ch. 16 - Prob. 6P Ch. 16 - Prob. 7P Ch. 16 - Prob. 8P Ch. 16 - Prob. 9P Ch. 16 - Prob. 10P

Solution Summary: The author analyzes the effects of glycolysis on the loss of allosteric site of ATP in phosphofructokinase (PFK).

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