Anatomy and Physiology: An Integrative Approach with Connect Access Card
3rd Edition
ISBN: 9781260254440
Author: Michael McKinley, Valerie O'Loughlin, Theresa Bidle
Publisher: McGraw-Hill Education
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Question
Chapter 15.4, Problem 9WDL
Summary Introduction
To differentiate:
Between the prevertebral ganglia and the sympathetic trunk ganglia.
Concept introduction:
The motor component of the ANS (autonomic nervous system) is divided into the sympathetic division and the parasympathetic division. The sympathetic division is complex anatomically then the parasympathetic one. Sympathetic division is also called as the thoracolumbar division.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 15 Solutions
Anatomy and Physiology: An Integrative Approach with Connect Access Card
Ch. 15.1 - LEARNING OBJECTIVE
1. List the similarities and...Ch. 15.1 - What criterion is used to organize the nervous...Ch. 15.1 - Prob. 2LOCh. 15.1 - Prob. 3LOCh. 15.1 - Prob. 2WDLCh. 15.1 - Prob. 4LOCh. 15.1 - What CNS structure is the integration and command...Ch. 15.2 - Prob. 5LOCh. 15.2 - Prob. 4WDLCh. 15.2 - Prob. 6LO
Ch. 15.2 - Describe the general anatomic differences in the...Ch. 15.2 - Prob. 7LOCh. 15.2 - Prob. 6WDLCh. 15.3 - Prob. 8LOCh. 15.3 - Prob. 1WDTCh. 15.3 - Which four cranial nerves have a parasympathetic...Ch. 15.3 - Prob. 9LOCh. 15.3 - What organs are innervated by the pelvic...Ch. 15.4 - Prob. 10LOCh. 15.4 - Prob. 11LOCh. 15.4 - Prob. 12LOCh. 15.4 - Prob. 13LOCh. 15.4 - Prob. 9WDLCh. 15.4 - Prob. 10WDLCh. 15.4 - Prob. 14LOCh. 15.4 - Prob. 15LOCh. 15.4 - Prob. 2WDTCh. 15.4 - Prob. 11WDLCh. 15.4 - In what ways does the adrenal medulla pathway help...Ch. 15.5 - Prob. 16LOCh. 15.5 - Prob. 17LOCh. 15.5 - Prob. 13WDLCh. 15.5 - Prob. 14WDLCh. 15.6 - Prob. 18LOCh. 15.6 - Prob. 15WDLCh. 15.6 - Prob. 19LOCh. 15.6 - Prob. 3WDTCh. 15.6 - Prob. 16WDLCh. 15.6 - Prob. 17WDLCh. 15.6 - Prob. 20LOCh. 15.6 - Prob. 21LOCh. 15.6 - Prob. 18WDLCh. 15.6 - Prob. 19WDLCh. 15.7 - LEARNING OBJECTIVE
22. Discuss the nature of...Ch. 15.7 - Prob. 20WDLCh. 15.7 - Prob. 23LOCh. 15.7 - LEARNING OBJECTIVE
24. Describe the antagonistic...Ch. 15.7 - Prob. 21WDLCh. 15.7 - Prob. 22WDLCh. 15.7 - Prob. 25LOCh. 15.7 - What are the body structures innervated by the...Ch. 15.8 - Prob. 26LOCh. 15.8 - 27. Describe some major examples of autonomic...Ch. 15.8 - Prob. 24WDLCh. 15 - A splanchnic nerve in the sympathetic division of...Ch. 15 - Some parasympathetic preganglionic neuron cell...Ch. 15 - Prob. 3DYBCh. 15 - Prob. 4DYBCh. 15 - Sympathetic division preganglionic axons travel to...Ch. 15 - Prob. 6DYBCh. 15 - Prob. 7DYBCh. 15 - A sympathetic postganglionic axon is a. long and...Ch. 15 - Prob. 9DYBCh. 15 - Prob. 10DYBCh. 15 - Prob. 11DYBCh. 15 - For the following ganglia, identify the location...Ch. 15 - Prob. 13DYBCh. 15 - Prob. 14DYBCh. 15 - Prob. 15DYBCh. 15 - Prob. 16DYBCh. 15 - Prob. 17DYBCh. 15 - What may occur with the mass activation of the...Ch. 15 - Prob. 19DYBCh. 15 - Prob. 20DYBCh. 15 - Prob. 1CALCh. 15 - Arlene was in a heightened state of alertness well...Ch. 15 - George has hypertension (high blood pressure). His...Ch. 15 - Prob. 4CALCh. 15 - Prob. 5CALCh. 15 - Prob. 1CSLCh. 15 - When you were younger, your parents may have told...Ch. 15 - Prob. 3CSL
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