EBK BIOLOGY
11th Edition
ISBN: 8220106820636
Author: Martin
Publisher: CENGAGE L
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Chapter 15.1, Problem 1LO
Summary Introduction
To explain: How a typical restriction enzyme cuts DNA molecules and give examples of their usage in recombinant DNA technology.
Concept introduction: DNA is a double-stranded molecule consisting of two strands of
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Briefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.
Describe how restriction endonuclease enzymes are used in recombinant dna technology
Explain how recombinant DNA technology uses restriction enzymes (restriction endonucleases).
Explain how recombinant DNA technology uses DNA ligase enzymes.
Chapter 15 Solutions
EBK BIOLOGY
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- How can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forwardDescribe the function of restriction enzymes and ligase in genetic recombination technology.arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forward
- Transgenic bacteria can be used to make an alanine rich (GM) plant. Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your explanation will include the role of: Restriction enzymes, plasmids, recombinant DNA, and bacteria.arrow_forwardDescribe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular cloning and genetic engineering.arrow_forwardExplain what restriction enzymes are, how they function and how they can be used to make recombinant DNAarrow_forward
- Briefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forwardName the five key tools for accomplishing the tasks of recombinant DNA technology.also mention the function of each tool.arrow_forwardOutline the steps in automated DNA sequencing via the dideoxy method.arrow_forward
- Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the DNA that are left. Why do we need to run a gel electrophoresis after enzyme digestionarrow_forwarda) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forwardRestriction endonuclease and ligase are two types of enzymes used in the process of genetic engineering, i.e., the manipulation of genes. The restriction endonuclease differs from ligase in that it breaks the DNA at ends, while ligase causes the breaks in DNA from interior joins the fragments of DNA, while ligase breaks the DNA into fragments breaks the DNA at specific points, while the ligase joins the fragments of DNA breaks the DNA apart at each nucleotide, while ligase use the pieces to translatearrow_forward
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