EBK BIOLOGY
11th Edition
ISBN: 8220106820636
Author: Martin
Publisher: CENGAGE L
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 15, Problem 9TYU
A CRISPR locus in a bacterium contains (a) short repeated sequences (b) short DNA sequences homologous to bacteriophages (c) genes that encode guide RNAs and one or more proteins (d) a, b, and c (e) none of the preceding
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Short tandem repeat sequences (STRs) are
a) used for DNA profiles
b) repeat sequences present in the human genome
c) highly variable in copy number
d) all of these
e) none of these
A scientist used a transgenic strain of C. elegans into
which a gene (gfp) for a green fluorescent protein had
been introduced. He injected some worms with
double-stranded RNA complementary to coding
sequence of the gfp gene. The figure below shows the
worms without (a; left) and with (b; right) ds gfp RNA.
a
(-) ds gfp RNA
_(+) ds gfp RNA
a) Explain these results .
b) The scientist conducted another experiment in which
he injected double-stranded RNA complementary to
the introns and promoter sequences of the gfp gene.
What results would you expect with this experiment?
Explain your answer .
What is an open-reading frame?
a) An unknown gene
b) The protein-encoding sequence of a gene
O c) A gene that no longer has a function
d) A non-translated region on the chromosome
Chapter 15 Solutions
EBK BIOLOGY
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Eukaryotic cells contain O a) 5 nuclear RNA polymerases b) 3 nuclear RNA polymerases O c) 6 nuclear RNA polymerases O d) none of these is true that transcribe distinct classes of genes.arrow_forwardWhich of the following is not true of cDNA produced using human brain tissue as the starting material? (A) It can be amplified by the polymerase chain reaction. (B) It was produced from pre-mRNA using reverse transcriptase. (C) It can be labeled and used as a probe to detect genes expressed in the brain. (D) It lacks the introns of the premRNAarrow_forwardYou would expect to find a sequence corresponding to the 5' untranslated region (5'-UTR) of a gene in A.) both a genomic DNA library and a cDNA library B.)a genomic DNA library C.)a cDNA library D.)neither a genomic DNA library nor a cDNA libraryarrow_forward
- The dideoxynucleosides ddATP, ddTTP, ddGTP, and ddCTP were important in DNA sequencing because they (a) cause premature termination of a growing DNA strand (b) are used as primers (c) cause the DNA fragments that contain them to migrate more slowly through a sequencing gel (d) are notaffected by high temperatures (e) have more energy than deoxynucleotidesarrow_forwardWith respect to recombinant vaccines, it is CORRECT to state that: * (Only one statement is correct) (a) Protein subunit vaccines are formulated with the expression vector encoding the immunogenic protein or protein fragment (b) Recombinant carrier organisms are genetically modified to contain the pathogenic protein of the organism that will be the target of the vaccine (c) Nucleic acid vaccines are formulated with DNA or RNA molecules that, once internalized by cells, will generate recombinant proteins that will trigger the immune response against the desired pathogen (d) Unlike DNA vaccines, mRNA vaccines do not need to be internalized by cells, being recognized as foreign molecules to the organism that received them. (e) Laboratory-produced phages can be used as delivery tools in DNA vaccines, which will allow the expression of immunogenic proteins to the organism that received them (f) Pseudoviral particles (VLPs) are produced in cells that receive expression vectors…arrow_forwardthe most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA clonearrow_forward
- a) 5 nuclear RNA polymerases b) 3 nuclear RNA polymerases . c) 6 nuclear RNA polymerases d) none of these is true Eukaryotic cells contain ___________________ that transcribe distinct classes of genes.arrow_forwardThe protein neuroD is a critical human protein translated in the brain during development. The protein is 357 amino acids long. What is the length of the human neuroD *gene* sequence? a)357 nucleotides b)1071 nucleotides c)119 nucleotides d)You cannot determine the length of the gene from this information alone.arrow_forwardPolymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This technique had indeed facilitated research in various areas of molecular biology and genetics. You would like to amplify a particular gene fragment from the yeast genome using Polymerase Chain Reaction (PCR): (i) What is the major enzyme required to kick start this reaction? (ii) Besides the enzymes mentioned in Q2 (a)(), state three (3) other chemicals required for the amplification process to be carried out.arrow_forward
- Identify the single most correct choice: a) DNA replication is a non-conservative process. b) Peroxide and ozone can be used as antimicrobial agents and their mechanism of action is via oxidizing molecules inside the cells. c) Antibiotics can never be used as food preservatives. d) All of the above.arrow_forwardThe following DNA sequences found on the sense strand belong to the same eukaryotic gene: Sequence 1: 5'-GATTCAATAAAGCTCAGATCGCTCACGTCGCGACTC-3' Sequence 2: 5'-TCCGAGGTCACTAGATACTCGTCGATCGTATAAATG-3' a) Which sequence is likely to be found upstream from the coding sequence? Justify your answer. b) Which sequence is likely to be found downstream from the coding sequence? Justify your answer. c) Which sequence will not be transcribed into an mRNA transcript? Justify your answer.arrow_forwardWhich of the following is NOT true of transposition? A) a replicative form of transposition is possible for DNA-only transposition B) flanking direct repeats are a by-product of transposition formed at the transposition site C) inverted repeats are formed in all types of transposons D long-terminal repeats are found in the class of retrotransposons that resemble retrovirusesarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license