EBK BIOLOGY
11th Edition
ISBN: 8220106820636
Author: Martin
Publisher: CENGAGE L
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Chapter 15, Problem 7TYU
Summary Introduction
Concept introduction: DNA sequencing is used to determine the exact arrangement of the
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GENETICS
The modified "dye terminator method for DNA sequencing represented a major improvement over Sanger's original method because ( among other things) it does NOT require
a) a DNA primer
b) DNA polymerase
c) the use of four separate sequencing reactions for each template
d) the use of electrophoresis to separate DNA chains based on size
e) the used chemically modified dNTPs
f) all of the above
During PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)
Since DNA is a hydrophillicmoelcule, it cannot pass through cell membranes. Name and explain the technique with which the DNA is forced into (ii) a bacterial cell (ii) a plant cell (iii) an animal cell.
Chapter 15 Solutions
EBK BIOLOGY
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- Which is not a property of DNA polymerase? a) It requires a primer to begin synthesis b) It adds dNTPS only in a 5' to 3' direction c) It opens the two strands of template DNA at the repliction fork d) Its exonuclease activity is used in proofreading. 6. In an analysis of the composition of double-stranded DNA, which will always be found? a) A = G b) G=T c) A+T= G+ C d) A+ G C+T e) A = C 7._ DNA is synthesized through a process that is a) conservative b) semi-discontinuous c) bi-directional d) both a and b e) both b and c 8. The role of DNA ligase is to a) stabilize unwound DNA b) join Okazaki fragments together c) synthesize the RNA primer d) unwind the double helix e) hold the polymerase-for-extension in place at the replication fork 9.arrow_forwardWhen joining two or more DNA fragments, a researcher can adjust the sequence at the junction in a variety of subtle ways, as seen in the following exercises.(a) Draw the structure of each end of a linear DNA fragment produced by an EcoRI restriction digest (include those sequences remaining from the EcoRI recognition sequence).(b) Draw the structure resulting from the reaction of this end sequence with DNA polymerase I and the four deoxynucleoside triphosphates.(c) Draw the sequence produced at the junction that arises if two ends with the structure derived in (b) are ligated (d) Draw the structure produced if the structure derived in (a) is treated with a nuclease that degrades only single-stranded DNA.(e) Draw the sequence of the junction produced if an end with structure (b) is ligated to an end with structure (d).(f) Draw the structure of the end of a linear DNA fragment that was produced by a PvuII restriction digest (include those sequences remaining from the PvuII recognition…arrow_forwardIn Polymerase Chain Reaction (PCR), the temperature is one of the most important parameters that could influence the efficiency of this technique. Each cycle of this reaction has its own specific temperature. For instance, the denaturation step possesses a temperature of 94 - 98 ℃ to ensure that the double stranded DNA is fully separated. (i) (ii) (iii) Why is the annealing temperature vital in this technique? Explain how will this temperature affects the efficiency of this reaction. Why is Hot Start PCR technique preferred by some researchers? If the primers you purchased possessed the following information. 5'-GGA AAC AGC TAT GAC CAT G-3' Calculate the melting temperature of this primer and estimate the annealing temperature of this primer.arrow_forward
- The following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many fragments were PRODUCED in DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forwardWhich one of the following options would be a good way to identify the location of the poly A tail in the published DNA sequence for a gene? Look for AATAAA near the 3’ UTR of the DNA sequence. Look for TTTTTTTTTTTTTT… (many Ts) near the 3’ UTR of the DNA sequence. Look for AAAAAAAAAAA… (many As) near the 3’ UTR of the DNA sequence. Look for a stop codon.arrow_forwardA team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (1) The sequence the scientists are interested in is 5-CATTGATTATTG[ 3-GTAACTAATAAG[ JATCAATTACGGG-3 JTAGTTAATGCCC-5 Where [...] indicates a longer 100bps sequence. Can you suggest two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid? ii) How can the scientist verify that their selection of primers was able to restrict the implementation to only that region of the plasmid?arrow_forward
- You prepare a reaction mix containing (i) DNA polymerase III, (ii) DATP, DCTP, dGTP, Mg2+, and 2,3'dideoxy-TTP (called ddTTP*), (iii) a short primer with the sequence 5'-CCTG-3, and (iv) a source DNA fragment with the sequence, 5'-AATCGTTCACGTTAGCAGG-3. What is the product of this reaction? Note that in ddTTP, both the 2' and 3' positions on the ribose sugar lack hydroxyl groups. No reaction, because the primer is not complementary to any sequence in the source DNA. O CCTGCT O CCTGC O T'T'AGCAAGT'GCAAT CGTCC O CCTGCT'AACGT GAACGAT'Tarrow_forwardThere may be more than one correct answer for each Repetitive DNA include which of the following? a.) SINEs (e.g. Alu) b.) microsatellite DNA c.) duplicated or diverged genes d.) long-interspersed nuclear elements DNA polymerases share which of the following characteristics? a.) They only add amino acids to an RNA primer. b.) They only add nucleotides to an existing strand or primer. c.) is the "string" of the "beads-on-a-string" appearance of condensed DNA d.) can be modified by acetylation or methylation to regulate DNA condensationarrow_forwardA team of scientist is interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (1) The sequence the scientists are interested in is 5-CATTGATTATTG 3-GTAACTAATAAG[ JATCAATTACGGG-3' JTAGTTAATGCCC-5 Where [...] indicates a longer 100bps sequence. Can you suggest two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid? i) How can the scientist verify that their selection of primers was able to restrict the implementation to only that region of the plasmid?arrow_forward
- In a Sanger DNA sequencing reaction (dideoxy method) you use the primer 5′-GCATATA-3′ to sequence a DNA strand that starts with the sequence 3′-CGTATATCCCTACGTTGG-5′ (consider the strand about 100-nucleotide long). You try your sequencing reaction three times, but every time you make a mistake, as indicated below. What would be the outcome in each case? Justify your answers. (a) You forgot to add dCTP (b) You forgot to add ddCTP (c) Your primer is complementary to two regions in the DNA to be sequenced.arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forwardRestriction endonucleases (RE) are one of the most used enzymes in biotechnology for recombinant DNA experimentations. These enzymes consist of three major classes; I, II and III. (i) Why is RE Class II commonly utilized in the labs as compared to Class I and III? (ii) Why is RE referred as Restriction Endonuclease and not Restriction Exonuclease? (iii) Some REs are known as isoschizomers. Describe isoschizomers with an example.arrow_forward
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