The problems that might arise while producing eukaryotic protein in a bacterium and how the usage of transgenic plants or animals may help to solve these problems. Concept introduction: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector. This recombinant plasmid expresses the eukaryotic gene by synthesizing a large quantity of protein encoded by it. There are certain hurdles in this, as the gene expression in eukaryotes and prokaryotes is different.

Question

Want to see the full answer?

Answer 1

Question

Chapter 15, Problem 12TYU

Summary Introduction

To determine: The problems that might arise while producing eukaryotic protein in a bacterium and how the usage of transgenic plants or animals may help to solve these problems.

Concept introduction: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector. This recombinant plasmid expresses the eukaryotic gene by synthesizing a large quantity of protein encoded by it. There are certain hurdles in this, as the gene expression in eukaryotes and prokaryotes is different.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

engineered constructs that consist of hollow fibers are acting as synthetic capillaries, around which cells have been loaded. The cellular space around a single fiber can be modeled as if it were a Krogh tissue cylinder. Each fiber has an outside “capillary” radius of 100 µm and the “tissue” radius can be taken as 200 µm. The following values apply to the device:R0 = 20 µM/secaO2 = 1.35 µM/mmHgDO2,T = 1.67 x 10-5 cm2/secPO2,m = 4 x 10-3 cm/secInstead of blood inside the fibers, the oxygen transport and tissue consumption are being investigated by usingan aqueous solution saturated with pure oxygen. As a result, there is no mass transfer resistance in the synthetic“capillary”, only that due to the membrane itself. Rather than accounting for pO2 variations along the length ofthe fiber, use an average value in the “capillary” of 130 mmHg.Is the tissue fully oxygenated?

Molecular Biology Please help with question. thank you You are studying the expression of the lac operon. You have isolated mutants as described below. In the presence of glucose, explain/describe what would happen, for each mutant, to the expression of the lac operon when you add lactose AND what would happen when the bacteria has used up all of the lactose (if the mutant is able to use lactose).5. Mutations in the lac operator that strengthen the binding of the lac repressor 200 fold 6. Mutations in the promoter that prevent binding of RNA polymerase 7. Mutations in CRP/CAP protein that prevent binding of cAMP8. Mutations in sigma factor that prevent binding of sigma to core RNA polymerase

Molecular Biology Please help and there is an attached image. Thank you. A bacteria has a gene whose protein/enzyme product is involved with the synthesis of a lipid necessary for the synthesis of the cell membrane. Expression of this gene requires the binding of a protein (called ACT) to a control sequence (called INC) next to the promoter. A. Is the expression/regulation of this gene an example of induction or repression?Please explain:B. Is this expression/regulation an example of positive or negative control?C. When the lipid is supplied in the media, the expression of the enzyme is turned off.Describe one likely mechanism for how this “turn off” is accomplished.