To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway. Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

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Answer 1

Question

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

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Answer 3

Question

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

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Answer 4

Question

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

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Answer 5

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

Answer 6

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

Answer 7

Chapter 15, Problem 35CQ

(a)

Summary Introduction

To match: The enzyme galactose dehydrogenase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Galactose dehydrogenase is an enzyme that belongs to oxidoreductase family of enzyme. This especially acts on the CH-OH donor group.

Answer 8

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

Answer 9

(b)

Summary Introduction

To explain: The enzyme GDP-D-mannose-3, 5-epimerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme GDP-D-mannose-3, 5-epimerase is an enzyme of isomerase family.

Answer 10

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

Answer 11

(c)

Summary Introduction

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

Answer 12

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

Answer 13

(d)

Summary Introduction

To explain: The enzyme phosphomannose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose isomerase is the facilitating enzyme that interconverts two molecules.

Answer 14

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

Answer 15

(e)

Summary Introduction

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

Answer 16

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Answer 17

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Answer 18

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Answer 19

Ch. 15 - Prob. 1E Ch. 15 - Prob. 2E Ch. 15 - 3. The reversible reaction shown here is part of...Ch. 15 - 4. Step 4 of the pentose phosphate pathway...Ch. 15 - 5. The aldolase reaction can proceed in revere as...Ch. 15 - Prob. 6E Ch. 15 - 7. Identify the intermediate in the...Ch. 15 - 8. The compound you identified in Problem 7 is a...Ch. 15 - 9. The pyruvate ? lactate reaction in animals is...Ch. 15 - Prob. 10E

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway. Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway. Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.

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Chapter 15 Solutions

To explain: The enzyme phosphoglucose isomerase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: Ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. Phosphoglucose isomerase is the isomerase enzyme.

To explain: The enzyme phosphomannose mutase with reaction given for the ascorbic acid biosynthesis pathway.

Concept introduction: The ascorbic acid is also known as L-ascorbic acid, which is a vitamin C element as well as an antioxidant. The enzyme phosphomannose mutase is a catalytic enzyme.