FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME
FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME
5th Edition
ISBN: 9781119750192
Author: Voet
Publisher: WILEY
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Chapter 15, Problem 31CQ

(a)

Summary Introduction

To explain: The way how glycerol enters to the glycolytic pathway.

Concept introduction: The conversion of glucose to pyruvate includes various energetically favorable and unfavorable reactions. The total conversion process is known as glycolysis. This pathway is called the glycolytic pathway.

(b)

Summary Introduction

To explain: The ATP yield for the conversion of glycerol to pyruvate.

Concept introduction: Glycerol enters to the glycolytic pathway in the metabolism of fructose that occurs in liver. Fructose enters to the liver in form of fructose-1-phosphate. Fructose-1-phospahte then undergoes aldolase reaction in the presence of fructose-1-phosphate aldolase forms glyceraldehydes and dihydroxy-acetone phosphate (DHAP). Glyceraldehyde undergoes dehydrogenation process in the presence of alcohol dehydogenase forms glycerol.

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Fura-2 Fluorescence (Arbitrary Unit) 4500 4000 3500 3000 2500 2000 1500 1000 500 [Ca2+]=2970nM, 25°C [Ca2+] 2970nM, 4°C [Ca2+]=0.9nM, 25°C [Ca2+] = 0.9nM, 4°C 0 260 280 300 340 360 380 400 420 440 Wavelength (nm) ← < The figure on the LHS shows the excitation spectra of Fura-2 (Em = 510 nm) in 2 solutions with two different Ca2+ ion concentration as indicated. Except for temperature, the setting for excitation & signal acquisition was identical.< ப a) The unit in Y-axis is arbitrary (unspecified). Why? < < b) Compare & contrast the excitation wavelength of the Isosbestic Point of Fura-2 at 25 °C & 4 °C. Give a possible reason for the discrepancy. < c) The fluorescence intensity at 25 °C & 4 °C are different. Explain why with the concept of electronic configuration. <

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FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME

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