Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 15, Problem 16P

The restriction enzymes Xho I andSal I cut their specific sequences as shown below:

Xho I 5'- C TCGAG -3'

3'- GAGCT C -5'

Sal I 5'- G TCGAC -3'

3'- CAGCT G -5'

Can the sticky ends created byXho I and Sal I sites be ligated? If yes, can the resulting sequences be cleaved by either Xho I or Sal I ?

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#16) The restriction enzymes Xhol and SalI cut their specific sequences as shown below:  XhoI 5' C | TCGAG 3'          SalI  | 5' GTCGAC 3'         3' GAGC | TC 5'                3' G | AGCTG 5'  Can the sticky ends created by XhoI and SalI sites be ligated? If yes, can the resulting sequences be cleaved by either XhoI or SalI?
You are studying a protein that contains the peptide sequence RDGSWKLVI. The part of the DNA encoding this peptide is included in the sequence shown below. 5'-CGTGACGGCTCGTGGAAGCTAGTCATC-3' 3'-GCACTGCCGAGCACCTTCGATCAGTAG-5' This sequence does not contain any BamHI restriction enzyme sites. The target sequence for the BamHI restriction nuclease is GGATCC. Your goal is to create a BamHI site on this plasmid by manipulating the DNA sequence, without changing the coding sequence of the protein. How would you do this, ie what would the new sequence be?
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Genetic Analysis: An Integrated Approach (3rd Edition)

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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License