Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 15, Problem 23P

You have generated three transgenic lines of maize that are resistant to the European corn borer, a significant pest in many regions of the world. The transgenic lines (T 1 in the accompanying table) were created using Agrobacteriummediated transformation with a T - DNA having two genes, the first being a gene conferring resistance to the corn borer and the second being a gene conferring resistance to a herbicide that you used as a selectable marker to obtain your transgenic plants. You crossed each of the lines to a wild - type maize plant and also generated a T 2 population by self - fertilization of the T 1 plant. The following segregation results were observed (herbicide resistant: herbicide sensitive):

Chapter 15, Problem 23P, You have generated three transgenic lines of maize that are resistant to the European corn borer, a

Explain these segregation ratios.

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Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…
Linkage in common bean Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population…
Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…

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Genetic Analysis: An Integrated Approach (3rd Edition)

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